Abstract
Leaf segments excised from Centella asiatica, a medicinal and neutraceutical plant, produced abundant somaticembryoswhen cultured onMS mediumwith 9.29 μMkinetin in combination with 2.26 μM2,4-D. Granular, white,shiny clusters of callus developed after 1 week of culture, and then formed heart and cotyledonary stage embryoson the same medium after 4 weeks. Somatic embryos matured and germinated in the presence of MS mediumcontaining 2.32 μM kinetin with (2.89μM) GA3. Plantlets were successfully transferred to pots containing amixture of soil and vermiculite (1:1).
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References
Ahmad Ru (1993) Medicinal plants used in ISM -- their procurement, cultivation, regeneration and import/export aspects: a review. In: Govil JN, Singh VK & Hashmi S (eds) Medicinal Plants: New Vistas of Research. Part I (pp 221–258). Today and Tomorrow printers and publishers, New Delhi.
Banerjee S, Zehra M & Kumar S (1999) In vitro multiplication of Centella asiatica, a medicinal herb from leaf explants. Curr. Sci. 76: 147–148.
Kameshwara Rao C (ed) (2000) In: Material for the Database of Medicinal Plants. Karnataka State council for Science and Technology for the Dept. of Forests, Environment and Ecology Govt. of Karnataka. 90 pp.
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant 15: 473–497.
Pandey NK, Tewari KC, Tewari RN, Joshi GC, Pande VN & Pandey G (1993). Medicinal plants of Kumaon Himalaya strategies for conservation. In: Dhar U (ed) Himalayan Biodiversity Conservation Strategies, No. 3 (pp 293–302) Himavikas Publications, Nanital.
Patra A, Rai B, Rout GR & Das P (1998) Successful plant regeneration from callus cultures of Centella asiatica (Linn.) Urban. Plant Growth Regul. 24: 13–16.
Prakash E, Shavalli Khan, Elusing Meru & Rao KR (2001) Somatic embryogenesis in Pimpinella tirupatiensis Bal and Subr., an endangered medicinal plant of Tirumala hills. Curr. Sci. 81(9): 1239–1242.
Samantaray S, Rout GR & Das P (1997) Regeneration of plants via somatic embryogenesis from leaf base and leaf tip segments of Echinochloa colona. Plant Cell Tiss. Org. Cult. 47: 119–125.
Sharma BL & Kumar A (1998) Biodiversity of Medicinal plants of Trigugi Narain (Garhwal Himalaya) and their conservation. National Conference on Recent Trends in Spices and Medicinal Plant Research, A-78 (2–4) April, Calcutta, WB, India.
Singh HG (1989) Himalayan herbs and drugs importance and extinction threat. J. Sci. Res. Plants Med. 10: 47–52.
Srivastava R, Shukla YN & Kumar S (1998) Chemistry and pharmacology of Centella asiatica: a review. J. Med. Arom. Plant Sci. 19: 1049–1056.
Tanaka K, Kanno Y, Kudo S & Suzuki M (2000) Somatic embryogenesis and plant regeneration in Chrysanthemum (Dendanthema grandiflorum (Ramt.) Kitamura). Plant Cell Rep. 19: 946–953.
Tiwari V, Singh BD & Tiwari KN (1998) Shoot regeneration and somatic embryogenesis from different explants of brahmi (Bacopa monniera L. wettst). Plant Cell Rep. 17: 538–543.
Tiwari KN, Sharma NC, Tivari V & Singh BD (2000) Micropropagation of Centella asiatica (L.), a valuable medicinal herb. Plant Cell Tiss. Org. Cult. 63: 179–185.
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Paramageetham, C., Prasad Babu, G. & Rao, J. Somatic embryogenesis in Centella asiatica L. an important medicinal and neutraceutical plant of India. Plant Cell, Tissue and Organ Culture 79, 19–24 (2004). https://doi.org/10.1023/B:TICU.0000049427.27937.d2
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DOI: https://doi.org/10.1023/B:TICU.0000049427.27937.d2