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In Vitro Culture of Kodo Millet: Influence of 2,4-D and Picloram in Combination with Kinetin on Callus Initiation and Regeneration

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Abstract

Immature inflorescences of kodo millet (Paspalum scrobiculatum L. cv. GPUK-3) were cultured on MS medium. Induction of embryogenic callus and subsequent somatic embryogenesis was possible on both 2,4-D and Picloram alone or with kinetin from spikelets as well as rachis. Immature inflorescence cultured on medium supplemented with lower levels of Picloram in combination with kinetin developed organogenic callus with shoot buds. Direct somatic embryo formation on rachis was observed at higher levels of Picloram in combination with kinetin. Plant regeneration was observed when calluses were transferred to α-napthaleneacetic acid (NAA) plus 6-benzylaminopurine (BA) supplemented MS medium. Histological observations provided a clear evidence for both somatic embryogenesis and shoot organogenesis. Profuse rooting was induced on phenylacetic acid (PAA) supplemented medium. Regenerated plants were successfully transferred to pots under field conditions where most of the plants survived and set normal seeds.

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Authors and Affiliations

  1. Department of Botany, University of Rajasthan, Jaipur, 302004, Rajasthan, India

    Preeti Kaur & S.L. Kothari

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  1. Preeti Kaur
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  2. S.L. Kothari
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Correspondence to S.L. Kothari.

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Kaur, P., Kothari, S. In Vitro Culture of Kodo Millet: Influence of 2,4-D and Picloram in Combination with Kinetin on Callus Initiation and Regeneration. Plant Cell, Tissue and Organ Culture 77, 73–79 (2004). https://doi.org/10.1023/B:TICU.0000016505.20448.44

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  • DOI: https://doi.org/10.1023/B:TICU.0000016505.20448.44

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