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Chemical and Chemoenzymatic Synthesis of Nucleoside 5′-α-Thiotriphosphates

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Abstract

New methods of chemical and chemoenzymatic synthesis of nucleoside 5′-thiophosphates and 5′-α-thiotriphosphates are developed. The 5′-α-thiotriphosphates are used as substrates both in template-dependent enzymatic PCR synthesis and in a T7–RNA transcription polymerase system.

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Authors and Affiliations

  1. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, GSP, Moscow, 117997, Russia

    K. V. Antonov, R. S. Esipov, A. I. Gurevich, D. V. Chuvikovsky, G. V. Mikulinskaya, S. A. Feofanov & A. I. Miroshnikov

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  1. K. V. Antonov
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  2. R. S. Esipov
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  3. A. I. Gurevich
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  4. D. V. Chuvikovsky
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  5. G. V. Mikulinskaya
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  6. S. A. Feofanov
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  7. A. I. Miroshnikov
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Antonov, K.V., Esipov, R.S., Gurevich, A.I. et al. Chemical and Chemoenzymatic Synthesis of Nucleoside 5′-α-Thiotriphosphates. Russian Journal of Bioorganic Chemistry 29, 560–565 (2003). https://doi.org/10.1023/B:RUBI.0000008897.08102.ee

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  • DOI: https://doi.org/10.1023/B:RUBI.0000008897.08102.ee

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