Abstract
The growth of the recombinant Bacillus subtilis strain AJ73 carrying the Bacillus intermedius 3-19 glutamyl endopeptidase gene on a multicopy plasmid and the effect of some nutrients on the efficiency of extracellular glutamyl endopeptidase production in the stationary growth phase were studied. In this phase, the concentration of glutamyl endopeptidase in the culture liquid peaked at the 48th and 78th hours of cultivation and depended on the composition of the cultivation medium. Unlike the synthesis of glutamyl endopeptidase in the trophophase (i.e., during vegetative growth), which was suppressed by glucose, the synthesis of this enzyme during sporulation was resistant to glucose present in the cultivation medium. A multifactorial experimental design allowed optimal proportions between the concentrations of major nutrients (peptone and inorganic phosphate) to be determined. Inorganic phosphate and ammonium ions augmented the production of glutamyl endopeptidase by 30–150%, and complex organic substrates, such as casein and gelatin, enhanced the production of glutamyl endopeptidase by 50–100%. During sporulation, the production of glutamyl endopeptidase was stimulated by some bivalent cations (Ca2+, Mg2+, and Co2+) and inhibited by others (Zn2+, Fe2+, and Cu2+). The inference is drawn that the regulatory mechanisms of glutamyl endopeptidase synthesis during vegetative growth and sporulation are different.
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REFERENCES
Rudenskaya, G.N., Microbial Glutamyl Endopeptidases, a New Subfamily of Chymotrypsin Proteinases, Bioorg. Khim., 1998, vol. 24,no. 4, pp. 256-261.
Leshchinskaya, I.B., Shakirov, E.V., Itskovitch, E.L., Balaban, N.P., Mardanova, A.M., Sharipova, M.R., Viryasov, M.B., Rudenskaya, G.N., and Stepanov, V.M., Glutamylendopeptidase of Bacillus intermedius Strain 3–19, FEBS Lett., 1997, vol. 404, pp. 241-244.
Rebrikov, D.V., Akimkina, T.V., Shevelev, A.B., Demiduyk, I.V., Bushueva, A.M., Kostrov, S.V., Chestukhina, G.G., and Stepanov, V.M., Molecular Cloning and Nucleotide Sequence of Bacillus intermedius Glutamylendopeptidase Gene, J. Prot. Chem., 1999, vol. 18, pp. 21-26.
Gabdrakhmanova, L.A., Shakirov, E.V., Balaban, N.P., Sharipova, M.R., Leshchinskaya, I.B., and Rudenskaya, G.N., Biosynthesis and Localization of Glutamylendopeptidase of Bacillus intermedius 3–19, Microbios, 1999, vol. 100, pp. 97-108.
Gabdrakhmanova, L.A., Balaban, N.P., Sharipova, M.R., Kostrov, S.V., Akimkina, T.V., Rudenskaya, G.N., and Leshchinskaya, I.B., Optimization of Bacillus intermedius Glutamylendopeptidase Production by Recombinant Strain of Bacillus subtilis and Localization of Glutamylendopeptidase in Bacillus subtilis Cells, Enzyme Microb. Technol., 2002, vol. 31, pp. 256-263.
Sharipova, M.R., Balaban, N.P., Gabdrakhmanova, L.A., Shilova, M.A., Kadyrova, Yu.M., Rudenskaya, G.N., and Leshchinskaya, I.B., Hydrolytic Enzymes and Sporulation in Bacillus intermedius, Mikrobiologiya, 2002, vol. 71,no. 4, pp. 494-499.
Klier, A., Msadek, T., and Rapoport, G., Positive Regulation in the Gram-Positive Bacterium Bacillus subtilis, Annu. Rev. Microbiol., 1992, vol. 46, pp. 429-459.
DNA Cloning: A Practical Approach, Glover, D.M., Ed., Oxford: IRL, 1985. Translated under the title Klonirovanie DNK. Metody, Moscow: Mir, 1988.
Gabdrakhmanova, L.A., Shakirov, E.V., Balaban, N.P., Sharipova, M.R., Rudenskaya, G.N., Kostrov, S.V., Akimkina, T.V., and Leshchinskaya, I.B., Optimized Medium for the Efficient Production of Bacillus intermedius Glutamyl Endopeptidase by the Recombinant Bacillus subtilis Strain AJ73, Mikrobiologiya, 2000, vol. 69,no. 5, pp. 653-659.
Rukovodstvo k Prakticheskim Zanyatiyam po Mikrobiologii (Workbook for Laboratory Classes in Microbiology), Egorov, N.S., Ed., Moscow: Mosk. Gos. Univ., 1983.
Plokhinskii, N.A., Matematicheskie Metody v Biologii (Mathematical Methods in Biology), Moscow: Mosk. Gos. Univ., 1978.
Biosintez mikroorganizmami nukleaz i proteaz (Biosynthesis of Nucleases and Proteases by Microorganisms), Imshenetskii, A.A., Ed., Moscow: Nauka, 1979.
Khaidarova, N.V., Rudenskaya, G.N., Revina, L.P., Stepanov, V.M., and Egorov, N.S., Glu,Asp-Specific Proteinase from Streptomyces thermovulgaris, Biokhimiya, 1989, vol. 54,no. 1, pp. 46-52.
Leshchinskaya, I.B., Shakirov, E.V., Itskovich, E.L., Balaban, N.P., Mardanova, A.M., Sharipova, M.R., Blagova, E.V., Levdikov, V.M., Kuranova, I.P., Rudenskaya, G.N., and Stepanov, V.M., Glutamyl Endopeptidase from Bacillus intermedius 3–19: Isolation, Properties, and Crystallization, Biokhimiya, 1997, vol. 62,no. 62, pp. 1052-1059.
Balaban, N.P., Mardanova, A.M., Sharipova, M.R., Gabdrakhmanova, L.A., Sokolova, E.A., Garusov, A.V., Mil'gotina, E.I., Rudenskaya, G.N., and Leshchinskaya, I.B., Isolation and Characterization of Glutamyl Endopeptidase 2 from Bacillus intermedius 3–19, Biokhimiya, 2003, vol. 68,no. 11, pp. 1514-1521.
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Chastukhina, I.B., Sharipova, M.R., Gabdrakhmanova, L.A. et al. The Regulation of Bacillus intermedius Glutamyl Endopeptidase Biosynthesis in the Recombinant Bacillus subtilis Strain AJ73 during Sporulation. Microbiology 73, 279–285 (2004). https://doi.org/10.1023/B:MICI.0000032237.91582.9b
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DOI: https://doi.org/10.1023/B:MICI.0000032237.91582.9b