Abstract
Establishment of microplants is related to the moisture vapourtransmission of the culture vessel lid. In this respect, stomatal andcuticular physiology were characterized in detached leaves from Dianthuscaryophyllus grown in the glasshouse or in vitro at different rates ofventilation. In vitro plants grown in non-ventilated culture vessels hadless waxes and therefore higher RWL compared to in vitro plants grown at Vr0.86 changes.h−1. The improvement of stomatal function inleaves obtained in ventilated vessels can be due to a performance of ionicrelations between guard and subsidiary cells, mainly by an increasingK+ concentration in the guard cells as ventilation decreases.Moreover, data showthat there is an increase in free ABA in the leavesfromventilated culture vessels to compensate for the conjugated ABA lostduring desiccation. If the proliferation stage proceeds in ventilatedculture vessels, the physiological characteristics of the plants producedare better than those obtained in non-ventilated culture vessels, confirmedby higher survival after soil transplantion.
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Boekestein A, Thiel F, Stols AL, Bouw E and Stadhouders, AM (1984) Surface roughness and the use of a peak to background ratio in the X-ray microanalysis of bioorganic bulk specimens. J. Microsc. 134: 327–333
Brainerd KE and Fuchigami LH (1982) Stomata functioning of in vitro and greenhouse apple leaves in darkness, mannitol, ABA and CO2. J. Exp. Bot. 33: 388–392
Brainerd KE, Fuchigami LH, Kwaitkowski S and Clark CS (1981) Leaf anatomy and water stress of aseptically cultured `Pixy' plum grown under different environments. HortScience 16: 173–175
Cassells AC and Wallsh C (1994) The influence of gas permeability of the culture lid on calcium uptake and stomata) function in Dianthus caryophyllus. Plant Cell Tissue Organ Cult. 37:171–178
Conner LN and Conner AJ (1984) Comparative water loss from leaves of Solanum laciniatum plants cultured in vitro and in vivo. Plant Sci. Lett. 36: 241–246
Diettrich B, Mertinat H and Luckner M (1992) Reduction of water loss during ex vitro acclimatization of micropropagated Digitalis lanata clone plants. Biochem. Physiol. Pflanz. 188: 23–31
Donnelly DJ, Vidaver WE and Lee KY (1985) The anatomy of tissue cultured red raspberry prior to and after transfer to soil. Plant Cell Tissue Organ Cult. 4: 43–49
Fuchigami LH, Cheng TY and Soeldner A (1981) Abaxial transpiration and water loss in aseptically cultured plum. J. Amer. Sue. Hort. Sci. 106: 519–522
Goldstein JI, Newbury DE, Echlin P, Joy DC and Lifshin E (eds) (1992) Scanning Electron Microscopy and X-ray Microanalysis. New York: Plenum Press
Grout BWW (1975) Wax development on leaf surfaces of Brassica oleracea var. Currawong regenerated from meristems culture. Plant Sci. Lett. 5: 401–405
Grout BW W and Aston MJ (1977) Transplanting of cauliflower plants regenerated from meristem culture. I. Water loss and water transfer related to changes in leaf wax and to xylem regeneration. Hortic. Res. 17: 1–7
Henson IE (1984) Evidence of a role for abscisic acid in mediating stomata] closure induced by obstructing translocation from leaves of Pearl millet (Pennisetum americanum L. Leeke). J. Exp. Bot. 35: 1419–1432
Jackson MB and Hall KC (1987) Early stomata closure in waterlogged pea plants is mediated by abscisic acid in the presence of foliar water deficits. Plant Cell Environ. 10: 121–130
Kersetiens G (1994) Effects of low light intensity and high air humidity on morphology and permeability of plant cuticles with special respect to plants cultured in vitro. In: Lumsden PJ, Nicholas JR and Davies WJ (eds) Physiology Growth and Development of Plants in Culture, pp 132–142. Dordrecht: Kluwer Academic Publishers
Kozai T and Smith MAL (1995) Environmental control in plant tissue culture. In Aitken-Christie J., Kozai T and Smith MAL (eds) Automation and Environmental Control in Plant Tissue Culture, pp 301–318. Dordrecht: Kluwer Academic Publishers
Kozai T, Fujiwara K and Watanabe I (1986) Fundamental studies on environments in plant tissue culture vessels. Effects of stoppers and vessels on gas exchange rates between inside and outside of vessels closed with stoppers. J. Agr. Met. 42: 119–127
Liu Z and Dickmann D (1992) Abscisic acid accumulation in leaves of two contrasting hybrid poplar affected by nitrogen fertilization plus cyclic flooding and soil drying. Tree Physiol. 11: 109–122
Majada JP (1995) Environmental Control and Automation of Vitroponic Cultures. PhD thesis. Oviedo University, Oviedo, Spain
Majada JP, Fal MA and Sánchez-Tames R (1997) The effect of ventilation rate on proliferation and hyperhydricity of Dianthus caryophyllus L. In vitro Cell. Dev. Biol. 33: 66–69
Marin JA, Gella R and Herrero M (1988) Stomatal structure and functioning as a response to environmental changes in acclimatized micropropagated Prunus cerasus L. Ann. Bot. 62:663–670
Markhart AH and Läuchli A (1982) The comparison of three freezing methods for electron probe X-ray microanalysis of hydrated barley root tissues. Plant Sci. Lett. 25: 29–36
Neill SJ and Horgan R (1987) Abscisic acid and related compounds. In: Rivier L and Crozier A (eds) The Principles and Practice of Plant Hormone Analysis. London: Plenum Press
Pasqualetto PL, Zimmerman RH and Fordham l (1988) The influence of cation and gelling agent concentrations on vitrification of apple cultivars in vitro. Plant Cell Tissue Organ Cult. 14: 31–40
Preece JE and Sutter EG (1991) Acclimatization of micropropagated plants to the greenhouse and field. In: Debergh PC and Zimmerman RH (eds) Micropropagation: Technology and Application, pp 71–93. Dordrecht: Kluwer Academic Publishers
Radin JW and Hendñx DL (1988) The apoplastic pool of abscisic acid in cotton leaves in relation to stomatal closure. Planta 174: 180–186
Radin JW, Parker LL and Guinn G. (1982) Water relations of cotton plants under nitrogen deficiency. V. Environmental control of abscisic acid accumulation and stomatal sensitivity to abscisic acid. Plant Physiol. 70: 1066–1070
Raschke K (1987) Action of ABA on guard cells. In: Zeiger E, Farquhar GD and Cowan IR (eds) Stomatal Function, pp 91–114. Stanford: Stanford University Press
Robinson DG, Ehlers U, Herken R, Herrmam BH, Mayer F and Scharmam FW (1987) Methods for SEM. In: Milhlethaler K (ed) Methods of Preparation for Electron Microscopy, pp 145–172. Berlin: Springer Verlag
Sallanon H, Tort M. and Coudret A (1993) The ultrastructure of micropropagated and greenhouse rose plant stomata. Plant Cell Tissue Organ Cult. 32: 227–233
Santamaria JM and Kerstiens G. (1994) The lack of control of water loss in micropropagated plants is not related to poor cuticle development. Physiol. Plant. 91: 191–195
Santamaria JM, Davies WJ and Atkinson CJ (1993) Stomata of micropropagated Delphinium plants respond to ABA, CO2, light and water potential but fail to close fully. J. Exp. Bot. 44: 99–107
Schroeder JI, Hedrich R, Fernandez JM. (1984) Potassium selective single channels in guard cell protoplasts of Vicia faba. Nature 312: 361–362
Shackel KA, Novello V, Sutter EG (1990) Stomatal function and cuticular conductance on whole tissue cultured apple plants. J. Amer. Soc. Hort. Sci. 115: 468–472
Sutter E (1984) Chemical composition of epicuticular wax in cabbage plants grown in vitro. Can. J. Bot. 62: 74–77
Sutter E (1985) Morphological physical and chemical characteristics of epicuticular wax on ornamental plants regenerated in vitro. Ann. Bot. 55: 321–329
Sutter E (1988) Stomatal and cuticular water loss from apple cherry and sweetgum plants after removal from in vitro culture. J. Amer. Soc. Hort. Sci. 113: 234–238
Sutter E and Langhans RW (1982) Formation of epicuticular wax and its effect of water loss in cabbage plants regenerated from shoot-tip culture. Can. J. Bot. 60: 2896–2902
Tardieu F and Davies WJ (1992) Stomatal response to abscisic acid is a function of current plant water stress. Plant Physiol. 98:540–545
Wardle K, Quinlan A and Simpkins I (1979) Abscisic acid and the regulation of water loss in plantlets of Brassica oleracea L. var. botrytis regenerated through apical meñstem culture. Ann. Bot. 43: 745–752
Wardle K, Dobbs EB and Short KC (1983) In vitro acclimatization of aseptically cultured plantlets to humidity. J. Amer. Soc. Hort. Sci 108: 383–389
Wetzstein HY and Sommer HE (1983). Scanning electron microscopy of in vitro cultured Liquidambar styraciflua plantlets during acclimatization. J. Amer. Soc. Hort. Soc. 108: 475–480
Zhang J and Davies WJ (1987) Increased synthesis of ABA in partially dehydrated root tips and ABA transport from roots to leaves. J. Exp. Bot. 38: 2015–2023
Zhang J and Davies WJ (1989) Abscisic acid produced in dehydrating roots may enable the plant to measure the water status of the soil. Plant Cell Environ. 12: 73–81
Ziv M (1991) Vitrification: morphological and physiological disorders of in vitro plants. In: Debergh PC and Zimmerman RH (eds) Micropropagation: Technology and Application, pp 45–69. Dordrecht: Kluwer Academic Publishers
Ziv M, Schwartz A and Fleminger D (1987) Malfunctioning stomata in vitreous leaves of carnation (Dianthus caryophyllus) plants propagated in vitro, implications for hardening. Plant. Sci. 52: 127–134.
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Majada, J.P., Centeno, M.L., Feito, I. et al. Stomatal and cuticular traits on carnation tissue culture under different ventilation conditions. Plant Growth Regulation 25, 113–121 (1998). https://doi.org/10.1023/B:GROW.0000009706.70078.1c
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DOI: https://doi.org/10.1023/B:GROW.0000009706.70078.1c