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Improvement of Sperm Motility of Sex-Reversed Male Rainbow Trout, Oncorhynchus mykiss, by Incubation in High-pH Artificial Seminal Plasma

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Abstract

Changes in the motility time of spermatozoa collected from the testes and the sperm duct of normal and sex-reversed male (XX) rainbow trout in physiological balanced salt solution were examined after incubation in artificial seminal plasmas of various pHs. Although untreated spermatozoa from the sperm duct retained motility for 60–90 s in the balanced salt solution, the spermatozoa collected from the testes were immotile. During the incubation in artificial seminal plasma of pH 7.0, the spermatozoa from the sperm duct hardly moved, similar to the testicular spermatozoa in the balanced salt solution. By suspending and incubating the testicular spermatozoa in artificial seminal plasma of pH 9.9 for 2 h at 4°C, the percentage of motile spermatozoa increased from 0–5% to 80%. The spermatozoa remained motile for at least 2 min after long-term incubation (12 h). When the full-sib eggs were inseminated with untreated testicular spermatozoa or testicular sperm treated for 2 h at high pH, the percentage survival increased from 5.5% to 53.8% at the eyed stage due to the high-pH treatment. The incubation of the spermatozoa in high-pH artificial seminal plasma improved the motility of the spermatozoa from the testes of the sex-reversed male that had lost its sperm duct. By this treatment, it is possible to markedly increase the mass production efficiency of all-female or all-female triploid sterile progenies.

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Authors and Affiliations

  1. Laboratory for Aquatic Biology, Department of Fisheries, Faculty of Agriculture, Kinki University, Nakamachi, Nara, 631-8505, Japan

    Toru Kobayashi & Koichi Ueno

  2. Fisheries Laboratory, Kinki University, Takada, Shingu, Wakayama, 647-1101, Japan

    Shozo Fushiki

Authors
  1. Toru Kobayashi
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  2. Shozo Fushiki
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  3. Koichi Ueno
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Kobayashi, T., Fushiki, S. & Ueno, K. Improvement of Sperm Motility of Sex-Reversed Male Rainbow Trout, Oncorhynchus mykiss, by Incubation in High-pH Artificial Seminal Plasma. Environmental Biology of Fishes 69, 419–425 (2004). https://doi.org/10.1023/B:EBFI.0000022904.35065.e8

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