Abstract
1. Sucrose feeding increases the level of stress-induced heat shock protein 70 mRNA in the rat hypothalamus. However, the mechanism by which a sucrose diet induces mRNA remains unclear. The issues investigated in this study were (1) whether a sucrose diet affects nitric oxide production in the hypothalamus, and (2) whether nitric oxide mediates the sucrose and stress-induced elevation of heat shock protein 70 mRNA.
2. To address the first question, we measured the level of nitrate, a final nitric-oxide-oxidation product measurable in vivo, using a microdialysis method. To address the second question, we administered a nitric oxide synthase inhibitor, N G-nitro-L-arginine methyl ester, prior to stress, then measured the mRNA level of heat shock protein 70 by the reverse transcription-competitive polymerase chain reaction method.
3. After the initiation of restraint stress, rats fed a sucrose-containing diet, unlike those fed standard chow, displayed a transient nitrate elevation. This nitrate elevation was attenuated by pretreatment with N G-nitro-L-arginine methyl ester. The mRNA level increases in rats fed a sucrose diet were dose-dependently attenuated by N G-nitro-L-arginine methyl ester.
4.These data suggest that a sucrose diet induces heat shock protein 70 under stress by enhancing nitric oxide production in the hypothalamus.
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Suzuki, E., Kageyama, H., Nakaki, T. et al. Nitric Oxide Induced Heat Shock Protein 70 mRNA in Rat Hypothalamus During Acute Restraint Stress Under Sucrose Diet. Cell Mol Neurobiol 23, 907–915 (2003). https://doi.org/10.1023/B:CEMN.0000005319.24621.73
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DOI: https://doi.org/10.1023/B:CEMN.0000005319.24621.73