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Interaction of Polymer Aggregates Based on Stearoyl-poly-N-vinylpyrrolidone with Blood Components

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Abstract

Stearoyl-poly-N-vinylpyrrolidone (PVP-stear) of various molecular weights (Mn = 1500-5500) self-assemble in aqueous medium. Particles prepared from PVP-stear were characterized in terms of shape and size distribution, and the mechanical stability of the particles was studied. The interaction of PVP-stear and its aggregates with blood components was investigated. Aggregates formed by the polymers with Mn = 1500-3500 in the presence of human serum are stable. The direct lytic action of PVP-stear preparations was studied using sheep and human erythrocytes. The influence of PVP-stear aggregates on the activation of complement system both on classical and alternative pathways was examined. The aggregates prepared from PVP-stear of various molecular weights had no effect on the activation of the complement system.

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REFERENCES

  1. Liaw, J., Aoyagi, T., Kataoka, K., Sakurai, Y., and Okano, T. (1998) Pharm. Res., 15, 1721–1726.

    Google Scholar 

  2. Yasugi, K., Nakamura, T., Nagasaki, Y., Kato, M., and Kataoka, K. (1999) J. Macromol., 32, 8024–8032.

    Google Scholar 

  3. Jeong, Y.-I., Nah, J.-W., Lee, Y.-K., Kim, S.-H., and Cho, C.-S. (1999) J. Controll. Rel., 188, 49–58.

    Google Scholar 

  4. Gref, R., Minamitake, Y., Peracchia, M. T., Trubetskoy, V. S., Torchilin, V. P., and Langer, R. (1994) Science, 263, 1600–1603.

    Google Scholar 

  5. Torchilin, V. P. (1988) J. Microencapsulation, 15, 1–19.

    Google Scholar 

  6. Torchilin, V. P. (2001) J. Controll. Rel., 73, 137–172.

    Google Scholar 

  7. Sidelskaya, F. P. (1970) Chemistry of N-Vinylpyrrolidone and Its Polymers [in Russian], Nauka, Moscow.

    Google Scholar 

  8. Shtilman, M. I., Michailova, E. V., Yaroslavov, A. A., and Torchilin, V. P. (1999) Biomaterials, 20, 280–285.

    Google Scholar 

  9. Cohen, S., and Bernstein, H. (1996) in Microparticulate Systems for the Delivery of Proteins and Vaccines (Cohen, S., ed.) Marcel Dekker, New York, pp. 1–49.

    Google Scholar 

  10. Roitt, A. (1991) Essential Immunology [Russian translation], Mir, Moscow.

    Google Scholar 

  11. Williams, C. A., and Chase, M. W. (1976) Methods in Immunology and Immunochemistry, Academic Press, New York.

    Google Scholar 

  12. Torchilin, V. P., Levchenko, T. S., Whiteman, K. R., Yaroslavov, A. A., Tsatsakis, A. M., Rizos, A. K., Michailova, E. V., and Shtilman, M. I. (2001) J. Biomaterials, 22, 3035–3044.

    Google Scholar 

  13. Kozlov, L. V., Krylova, Yu. I., Chikh, V. P., and Molchanova, P. N. (1982) Bioogr. Khim., 8, 652–659.

    Google Scholar 

  14. Raepple, E., Hill, H. U., and Loos, M. (1976) Immunochemistry, 13, 251–255.

    Google Scholar 

  15. Scherbak, I. G., Galebskaya, L. V., and Ryumina, E. V. (1992) RF Patent No. 1532876; Byul. Izobret., 14, 188.

    Google Scholar 

  16. Khalyapin, B. D., and Prokop'ev, A. A. (1986) Immunologiya, 3, 66–69.

    Google Scholar 

  17. Mittel, K. (1980) Micelle Formation, Solubilization and Microemulsions [Russian translation], Mir, Moscow.

    Google Scholar 

  18. Zhang, L., and Eisenberg, A. (1995) Science, 268, 1728–1731.

    Google Scholar 

  19. Tarasova, Yu. V. (2000) Blood Plasma Proteins as Regulators of Hemolysis: Candidate's dissertation [in Russian], St. Petersburg.

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Authors and Affiliations

  1. Faculty of Chemistry, Lomonosov Moscow State University, Moscow, 119992, Russia

    A. L. Villemson & N. I. Larionova

  2. Mendeleev University of Chemical Technology, Miusskaya pl. 9, Moscow, 125047, Russia

    A. N. Kuskov & M. I. Shtilman

  3. Pavlov St. Petersburg State Medical University, St. Petersburg, 197022, Russia

    L. V. Galebskaya & E. V. Ryumina

Authors
  1. A. L. Villemson
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  2. A. N. Kuskov
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  3. M. I. Shtilman
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  4. L. V. Galebskaya
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  5. E. V. Ryumina
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  6. N. I. Larionova
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Correspondence to N. I. Larionova.

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Villemson, A.L., Kuskov, A.N., Shtilman, M.I. et al. Interaction of Polymer Aggregates Based on Stearoyl-poly-N-vinylpyrrolidone with Blood Components. Biochemistry (Moscow) 69, 621–628 (2004). https://doi.org/10.1023/B:BIRY.0000033735.34356.21

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  • DOI: https://doi.org/10.1023/B:BIRY.0000033735.34356.21

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