biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 48:613-615, 2004 | DOI: 10.1023/B:BIOP.0000047163.70240.69

Effect of NaCl on Biomass and Contents of Sugars, Proline and Proteins in Seedlings and Leaf Explants of Nicotiana tabacum Grown in vitro

V. Niknam1, M. Bagherzadeh1, H. Ebrahimzadeh1, A. Sokhansanj1
1 Department of Biology, Faculty of Sciences, University of Tehran, Tehran, I.R. Iran

Effects of NaCl on growth in vitro and contents of sugars, free proline and proteins in the seedlings and leaf explants of Nicotiana tabacum cv. Virginia were investigated. The fresh and dry mass of the seedlings decreased under salinity. These growth parameters in leaf explants decreased at 50 mM NaCl and increased up to 150 mM NaCl and then decreased at higher level of salinity. Free proline content in both seedlings and leaf explants increased and polysaccharide content decreased continuously with increasing of NaCl concentration. Reducing sugars, oligosaccharides, soluble sugars and total sugars contents in both seedlings and leaf explants decreased up to 150 mM NaCl and then increased at higher concentrations of NaCl.

Keywords: salt stress; tobacco; tissue culture
Subjects: auxins; cytokinins; germination; growth analysis, plant development, biomass and yield enhancement; growth, salinity; in vitro culture, from leaf segments; Nicotiana tabacum; nutrient medium, Murashige and Skoog; osmolytes, salt stress; photon flux density; polysaccharides, salinity; proline, salt stress; protein, salinity; saccharides, total, salinity; salt stress, sugars, proline, protein

Published: December 1, 2004  Show citation

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Niknam, V., Bagherzadeh, M., Ebrahimzadeh, H., & Sokhansanj, A. (2004). Effect of NaCl on Biomass and Contents of Sugars, Proline and Proteins in Seedlings and Leaf Explants of Nicotiana tabacum Grown in vitro. Biologia plantarum, 48(4), 613-615. doi: 10.1023/B:BIOP.0000047163.70240.69
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