Skip to main content
SpringerLink
Log in

Degradation of the Herbicide Atrazine by the Soil Mycelial Fungus INBI 2-26 (–), a Producer of Cellobiose Dehydrogenase

  • Published:
Applied Biochemistry and Microbiology Aims and scope Submit manuscript

Abstract

Nonsporulating mycelial fungi producing cellobiose dehydrogenase (CDH) and isolated from soils of South Vietnam with a high residual content of dioxins are capable of growing on a solid medium in the presence of high atrazine concentrations (to 500 mg/l). At 20 and 50 mg/l atrazine, the area of fungal colonies was 1.5–1.2-fold larger, respectively, than the control colonies of the same age, whereas development of the colonies at 500 mg/l atrazine was delayed by 5 days, compared with controls grown in the absence of atrazine. Surface cultivation of the fungus on a minimal medium with glucose as a sole source of carbon and energy decreased the initial concentration of atrazine (20 mg/l) 50 times in 40 days; in addition, no pronounced sorption of atrazine by mycelium was detected. This was paralleled by an accumulation in the culture medium of extracellular CDH; atrazine increased the synthesis of this enzyme two- to threefold. Accumulation of β-glucosidase (a mycelium-associated enzyme) and cellulases preceded the formation of CDH.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Jaskulke, E., Patty, L., and Bruchet, A., Pesticide Chemistry and Bioscience. The Food-Environment Challenge, Cambridge, UK: Royal Soc. Chem., 1999, pp. 368–385.

    Google Scholar 

  2. Barcello, D., Rev. Analyst, 1991, vol. 116, pp. 681–689.

    Google Scholar 

  3. Solomon, K.R., Pesticide Chemistry and Bioscience. The Food-Environment Challenge, Cambridge, UK: Royal Soc. Chem., 1999, pp. 313–326.

    Google Scholar 

  4. Tappe, W., Groeneweg, J., and Jantsch, B., Biodegradation, 2002, vol. 13, no. 3, p. 10.

    Google Scholar 

  5. DÖrfler, U., Scheunert, I., Viswanarhan, R., and Zsolnay, A., Standortgerechte Berwertung Von S-Triazinen in Boden, Berlin: Forschungszentrum fur Umwelt und Gesundheit, 1997, p. 89.

    Google Scholar 

  6. EC Directive, 1998, 98/83/EC no. L330.

  7. Lode, O., Eklo, O.M., Holen, B., Svenson, A., and Johnsen, A.M., Sci. Total Environ., 1995, vol. 160-161, pp. 421–431.

    Google Scholar 

  8. Barbash, J.E., Thelin, G.P., Koplin, D.W., and Gilliom, R.J., Distribution of Major Herbicides in Ground Water of the United States, Sacramento: Geol. Surv., 1999, pp. 125–135.

    Google Scholar 

  9. Blau, K., Holden, P.W., Johnson, L.C., and Cheung, M.W., Triazine Herbicides: Risk Assessment, Washington: Amer. Chem. Soc., 1998, pp. 227–238.

    Google Scholar 

  10. Wackett, L.P., Sadowsky, M.J., Martinez, B., and Shapir, N., Appl. Microbiol. Biotechnol., 2002, vol. 58, no. 1, pp. 39–45.

    Google Scholar 

  11. Bending, G.D., Friloux, M., and Walker, A., FEMS Microbiol. Lett., 2002, vol. 18, no. 1, pp. 59–63.

    Google Scholar 

  12. Masaphy, S., Henis, Y., and Levanon, D., Appl. Environ. Microbiol., 1996, vol. 62, no. 10, pp. 3587–3593.

    Google Scholar 

  13. Nishimura, K., Yamamoto, M., Nakagomi, T., Takiguchi, Y., Naganuma, T., and Uzuka, Y., Appl. Microbiol. Biotechnol., 2002, vol. 58, no. 6, pp. 848–852.

    Google Scholar 

  14. Bollag, J.-M., Myers, C.J., and Minard, R.D., Sci. Total Environ., 1992, vol. 12, pp. 205–217.

    Google Scholar 

  15. Vasil'chenko, L.G., Koroleva, O.V., Stepanova, E.V., Landesman, E.O., and Rabinovich, M.L., Prikl. Biokhim. Mikrobiol., 2000, vol. 36, no. 4, pp. 412–421.

    Google Scholar 

  16. Koroleva, O.V., Stepanova, E.V., Landesman, E.O., Vasilchenko, L.G., Khromonygina, V.V., Zherdev, A.V., and Rabinovich, M.L., Toxicol. Environ. Chem., 2001, vol. 80, no. 3-4, pp. 175–188.

    Google Scholar 

  17. Koroleva, O.V., Stepanova, E.V., Landesman, E.O., Vasil'chenko, L.G., Khromonygina, V.V., Zherdev, A.V., and Rabinovich, M.L., Prikl. Biokhim. Mikrobiol., 2002, vol. 38, no. 4, pp. 413–418.

    Google Scholar 

  18. Vasil'chenko, L.G., Khromonygina, V.V., Koroleva, O.V., Landesman, E.O., Gaponenko, V.V., Kovaleva, T.A., Kozlov, Yu.P., and Rabinovich, M.L., Prikl. Biokhim. Mikrobiol., 2002, vol. 38, no. 5, pp. 534–539.

    Google Scholar 

  19. Jensen Jr., K.A., Houtman, C.J., Ryan, Z.C., and Hammel, K.E., Appl. Environ. Microbiol., 2002, vol. 68, no. 6, pp. 2705–2711.

    Google Scholar 

  20. Fahr, K., Wetzstein, H.G., Grey, R., and Schlosser, D., FEMS Microbiol. Lett., 1999, vol. 175, no. 1, pp. 127–132.

    Google Scholar 

  21. Paszczynski, A., Crawford, D., Funk, L., and Goodell, B., Appl. Environ. Microbiol., 1999, vol. 65, pp. 674–679.

    Google Scholar 

  22. Hyde, S.M. and Wood, P.M., Microbiology, 1997, vol. 143, pp. 259–266.

    Google Scholar 

  23. Igarashi, K., Verhagen, M.F., Samejima, M., Schulein, M., Eriksson, K.E., and Nishino, T., J. Biol. Chem., 1999, vol. 274, no. 6, pp. 3338–3344.

    Google Scholar 

  24. Dumonceaux, T.J., Bartholomew, K.A., Charles, T.C., Moukha, S.M., and Archibald, F.S., Gene, 1998, vol. 210, no. 2, pp. 211–219.

    Google Scholar 

  25. Fang, J., Liu, W., and Gao, P.J., Arch. Biochem. Biophys., 1998, vol. 353, no. 1, pp. 37–46.

    Google Scholar 

  26. Moukha, S.M., Dumonceaux, T.J., Record, E., and Archibald, F.S., Gene, 1999, vol. 234, no. 1, pp. 23–33.

    Google Scholar 

  27. Subramaniam, S.S., Nagalla, S.R., and Renganathan, V., Arch. Biochem. Biophys., 1999, vol. 365, no. 2, pp. 223–230.

    Google Scholar 

  28. Ludwig, R. and Haltrich, D., Lett. Appl. Microbiol., 2003, vol. 61, no. 1, pp. 32–39.

    Google Scholar 

  29. Baminger, U., Subramaniam, S.S., Renganathan, V., and Haltrich, D., Appl. Environ. Microbiol., 2001, vol. 67, no. 4, pp. 1766–1774.

    Google Scholar 

  30. Dekker, R.H.F., Methods Enzymol., 1988, vol. 160, pp. 454–463.

    Google Scholar 

  31. Karapetyan, K.N., Yachkova, S.N., Vasil'chenko, L.G., Borzykh, M.N., and Rabinovich, M.L., Prikl. Biokhim. Mikrobiol., 2003, vol. 39, no. 6, pp. 652–651.

    Google Scholar 

  32. Rabinovich, M.L., Klesov, A.A., and Berezin, I.V., Bioorg. Khim., 1977, vol. 3, pp. 405–414.

    Google Scholar 

  33. Miller, M., Palojarvi., Rangger, A., Reeslev, M., and Kjoller, A., Appl. Environ. Microbiol., 1998, no. 2, pp. 613–617.

  34. Henriksson, G., Johansson, G., and Pettersson, G., J. Biotechnol., 2000, vol. 78, pp. 93–113.

    Google Scholar 

  35. Szabo, I.J., Johansson, G., and Pettersson, G., J. Biotechnol., 1996, vol. 48, pp. 221–230.

    Google Scholar 

  36. Hutterman, A. and Noelle, A., J. Microbiol. Biotechnol., 1982, vol. 13, no. 6, pp. 683–688.

    Google Scholar 

  37. Roy, B.P., Dumonnceaux, T., Koukoulas, A.A., and Archibald, F.S., Appl. Environ. Microbiol., 1996, vol. 62, no. 12, pp. 4417–4427.

    Google Scholar 

  38. Rabinovich, M.L., Mel'nik, M.S., and Bolobova, A.V., Struktura i mekhanizm deistviya tsellyuloliticheskikh fermentov. Biokhimiya, 2000, vol. 67, no. 8, pp. 1026–1050.

    Google Scholar 

  39. Cameron, M.D. and Aust, S.D., Arch. Biochem. Biophys., 1999, vol. 367, no. 1, pp. 115–121.

    Google Scholar 

  40. Igarashi, K., Samejima, M., Saburi, Y., Habu, N., and Eriksson, K.E., Fungal Genet. Biol., 1997, vol. 21, no. 2, pp. 214–222.

    Google Scholar 

  41. Ilmen, M., Saloheimo, A., Onnela, M.L., and Penttila, M.E., Appl. Environ. Microbiol., 1997, vol. 63, no. 4, pp. 1298–1306.

    Google Scholar 

  42. Kubicek, C.P., Messner, R., Gruber, F., Mach, R.L., and Kubicek-Pranz, E.M., Enzym.Microb.Technol., 1993, vol. 15, no. 2, pp. 90–99.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Russian University of Peoples' Friendship, Moscow, 115093, Russia

    V. V. Khromonygina, A. I. Saltykova & Yu. P. Kozlov

  2. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr. 33, Moscow, 119071, Russia

    L. G. Vasil'chenko & M. L. Rabinovich

Authors
  1. V. V. Khromonygina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. I. Saltykova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. G. Vasil'chenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yu. P. Kozlov
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. L. Rabinovich
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Khromonygina, V.V., Saltykova, A.I., Vasil'chenko, L.G. et al. Degradation of the Herbicide Atrazine by the Soil Mycelial Fungus INBI 2-26 (–), a Producer of Cellobiose Dehydrogenase. Applied Biochemistry and Microbiology 40, 285–290 (2004). https://doi.org/10.1023/B:ABIM.0000025953.93099.a9

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/B:ABIM.0000025953.93099.a9

Keywords

Search

Navigation