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The Effect of Lr19-Translocation on in Vitro Androgenesis and Inheritance of Leaf-Rust Resistance in DH3 Lines and F2 Hybrids of Common Wheat

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Abstract

Leaf-rust resistance and androgenesis were studied in the anther cultures of Triticum aestivum L., which included Saratovskaya 29 cultivar, the isogenic line Ps29, and three F1 hybrids (L503/S55, L504/S58, ATS7/L1063) with 7DS-7DL-7Ae#1L translocation of Lr19 gene (Lr19 translocation) from Agropyron elongatum (Host.) P.B. The Lr19 translocation was shown to affect the induction of embryogenesis and green plant regeneration. The frequencies of Lr19 translocation differed in F2 hybrids obtained by traditional hybridization and in sets of DH3 lines obtained in F1 anther cultures derived from the same combinations of T. aestivum parental forms. The number of leaf-rust resistant genotypes tended to decrease. The frequency of Lr19 translocation in the set of DH3 lines derived from F1 L504/S58 was significantly lower than in other sets of DH3 lines and F2 hybrid populations.

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REFERENCES

  1. Devaux, P., Comparison of Anther Culture and the Hor-deum bulbosum Method for the Production of Doubled Haploids in Winter Barley, Plant Breed., 1988, vol. 100, pp. 181–187.

    Google Scholar 

  2. Liu, W., Zheng, M.Y., Polle, E.A., and Konzak, C.F., Highly Efficient Doubled-Haploid Production in Wheat (Triticum aestivum L.) via Induced Microspore Embryogenesis, Crop Sci., 2002, vol. 42, pp. 686–692.

    Google Scholar 

  3. Henry, Y. and De Buyser, J., Effect of the 1B/1R Trans-location on Anther Culture Ability in Wheat (Triticum aestivum), Plant Cell Rep., 1985, vol. 4, pp. 307–310.

    Google Scholar 

  4. Agache, S., Bachelier, D., De Buyser, J., et al., Studies on the Genetic Relationship between Anther Culture and Somatic Tissue Culture Abilities in Wheat, Plant Breed., 1988, vol. 100, pp. 26–33.

    Google Scholar 

  5. Agache, S., Bachelier, D., De Buyser, J., et al., Genetic Analysis of Anther Culture Response in Wheat Using Aneuploid, Chromosome Substitution and Translocation Lines, Theor. Appl. Genet., 1989, vol. 77, no. 2, pp. 7–11.

    Google Scholar 

  6. Sagi, L. and Barnabas, B., Evidences for Cytoplasmic Control of In Vitro Microspore Embryogenesis in Anther Culture of Wheat (Triticum aestivum L.), Theor. Appl. Genet., 1989, vol. 78, pp. 867–872.

    Google Scholar 

  7. Ekiz Hasan and Konzak, C.F., Nuclear and Cytoplasmic Control of Anther Culture Response in Wheat: 1. Analy-sis of Alloplasmatic Lines, Crop Sci., 1991, vol. 31, pp. 1421–1427.

    Google Scholar 

  8. Ekiz Hasan and Konzak, C.F., Nuclear and Cytoplasmic Control of Anther Culture Response in Wheat: 2. Com-monv Wheat x Alloplasmatic Lines, Crop Sci., 1991, vol. 31, pp. 1427–1431.

    Google Scholar 

  9. Andersen, S.D., Bue, I.K., and Olsen, A., The Response ofAnther Culture in a Genetically Wide Material of Win-ter Wheat (T. aestivum L.), Plant Breed., 1987, vol. 99, pp. 181–186.

    Google Scholar 

  10. Tuvenson, I.K.D., Pedersen, S., and Andersen, S., Nuclear Genes Affecting Albinism in Wheat (Triticum aestivum L.) Anther Culture, Theor. Appl. Genet., 1989, vol. 78, pp. 879–893.

    Google Scholar 

  11. Larsen, E.T., Tuvenson, I.K.D., and Andersen, S.B., Nuclear Genes Affecting Percentage of Green Plants in Barley (Hordeum vulgare L.) Anther Culture, Theor. Appl. Genet., 1991, vol. 82, pp. 417–420.

    Google Scholar 

  12. Torp, A.M., Hansen, A.L., Holme, I.B., and Andersen, S.B., Genetic Markers for Haploid Formation in Wheat Anther Culture, Proc. 9th Int. Gen. Symp., Saskatoon, Saskat-chewan, Canada, 1998, vol. 3, pp. 159–161.

    Google Scholar 

  13. Torp, A.M., Hansen, A.L., and Andersen, S.B., Chromosomal Regions Associated with Green Plant Regeneration in Wheat (Triticum aestivum L.) Anther Culture, Euphytica, 2001, vol. 119, pp. 377–387.

    Google Scholar 

  14. Zhang, L., Sourdille, P., Bernard, M., et al., QTL Map-ping for Anther Culturability in Wheat Using a Doubled-Haploid Population, Proc. 10th Int. Gen. Symp., Paestum, Italy, 2003, vol. 3, pp. 1078–1080.

    Google Scholar 

  15. Tkachenko, O.V., Djatchouk, T.I., and Lobachev, Yu.V., The Influence of Rht Genes on Wheat Anther Culture, Ann. Wheat Newslett., 1998, vol. 44, p. 169.

    Google Scholar 

  16. Djatchouk, T.I., Tkachenko, O.V., and Lobachev, Yu.V., The Effect of Rht Alleles on Wheat Anther Culture, Ann. Wheat Newslett., 1999, vol. 45, p. 123.

    Google Scholar 

  17. Djatchouk, T.I., Tkachenko, O.V., and Lobachev, Yu.V., Rht Genes Influence Androgenesis and Somatic Embryogenesis in Vitro in Spring Bread Wheat Anther, Ann. Wheat Newslett., 2001, vol. 47.

  18. Lane, R.U. and Mornhinweg, D., Inheritance of 1BL/1RS Wheat–Rye Translocated Chromosome in Wheat, Crop Sci., 1988, vol. 28, pp. 709–711.

    Google Scholar 

  19. Muller, G., Borschell, H., Vahl, V., et al., Die Nutzung der Antherenkulturmethode im Zuchtprozeß von Winter-weizen: 1. Zur Androgenesefähigkeit von 1B-1R–Wei-zen–Roggen–Translocationsformen, Plant Breed., 1989, vol. 102, pp. 196–207.

    Google Scholar 

  20. Muller, G., Bohme, T., Borschell, H., et al., Die Nutzung der Antherenkulturmethode in Zuchtprozeßvon Winter-weizen: 3. Zur Antherenkultureignung von Winterwei-zen F 1-Populationen mit den beiden heterozygoten Chro-mosomenpaaren 1AL-AS, 1AL-RS und 1BL-1RS/1RL-1RS, Plant Breed., 1990, vol. 104, pp. 272–280.

    Google Scholar 

  21. Dobrovolskaya, O.B., Pershina, L.A., Kravtzova, L.A., and Shchapova, A.I., The Peculiarities of Androgenesis in Wheat–Rye Substitution Lines, Proc. 11th EWAC Conf., Novosibirsk, Russia, 2000, pp. 105–107.

    Google Scholar 

  22. Dobrovolskaya, O.B., Characterization of Wheat–Rye Substitution Lines with the Use of Microsatellite Mark-ers and the Effects of Individual Rye Chromosomes on the Parameters of in Vitro Androgenesis, Cand. Sci. (Biol.) Dissertation, Novosibirsk, 2003.

  23. Sibikeeva, Yu.E. and Sibikeev, S.N., Genetic Analysis of Anther Culture Response in Wheat Carrying Alien Translocations, Theor. Appl. Genet., 1996, vol. 92, pp. 782–785.

    Google Scholar 

  24. Bernard, S., in Vitro Androgenesis in Hexaploid Triti-cale: Determination of Physical Condition Increasing Embryoid and Green Plant Production, Z. Pflanzen-zucht., 1980, vol. 85, pp. 308–321.

    Google Scholar 

  25. Chu, C.C., Establishment of an Efficient Medium for Anther Culture of Rice through Comparative Experi-ments on the Nitrogen Sources, Sci. Sinica, 1975, vol. 18, pp. 659–668.

    Google Scholar 

  26. Ouyang, J., Induction of Pollen Plants in Triticum aesti-vum, in Haploids of Higher Plants in Vitro, Berlin: Springer-Verlag, 1986, pp. 26–41.

    Google Scholar 

  27. Kibirige-Sebunya, I. and Knott, D.R., Transfer of Stem-Rust Resistance to Wheat from Agropyron Chromosome Having a Gametocidal Effect, Can. J. Genet. Cytol., 1983, vol. 25, pp. 215–221.

    Google Scholar 

  28. Marais, G.F., Preferential Transmission in Bread Wheat of a Chromosome Segment Derived from Thinopyrum distichum (Thunb.) Love., Plant Breed., 1990, vol. 104, pp. 152–159.

    Google Scholar 

  29. Zhang, H.B. and Dvorak, J., Characterization and Distri-bution of an Interspersed Repeated Nucleotide Sequence from Lophopyrum elongatum and Mapping of Segrega-tion-Distortion Factor with It, Genome, 1990, vol. 33, pp. 927–936.

    Google Scholar 

  30. Prins, R., Marais, G.F., Pretorius, Z.A., et al., A Study of Modified Forms of the Lr19 Translocation of Common Wheat, Theor. Appl. Genet., 1997, vol. 95, pp. 424–430.

    Google Scholar 

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  1. Agricultural Research Institute for Southeast Regions, Saratov, 410010, Russia

    Yu. E. Sibikeeva, S. N. Sibikeev & V. A. Krupnov

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  1. Yu. E. Sibikeeva
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  2. S. N. Sibikeev
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  3. V. A. Krupnov
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Sibikeeva, Y.E., Sibikeev, S.N. & Krupnov, V.A. The Effect of Lr19-Translocation on in Vitro Androgenesis and Inheritance of Leaf-Rust Resistance in DH3 Lines and F2 Hybrids of Common Wheat. Russian Journal of Genetics 40, 1003–1006 (2004). https://doi.org/10.1023/B:RUGE.0000041379.30508.39

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