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One-dimensional 13C NMR and HPLC-1H NMR techniques for observing carbon-13 and deuterium labelling in biosynthetic studies

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Abstract

For several decades isotope labelling techniques have been the indispensable tools used to unravel pathways of secondary product biosynthesis. NMR spectroscopy, together with mass spectrometry, is the most effective measuring technique used in the analysis of metabolites enriched with stable isotopes. 2H and 13C are the NMR-detectable nuclides which have been most frequently employed in plant secondary metabolite synthesis. Examples from the biosynthesis of phenylpropanoids, phenylphenalenones, and glucosinolates are used when discussing some aspects of one-dimensional NMR analysis of metabolites selectively labelled with 2H and 13C. Besides direct NMR detection of 13C-enriched metabolites, special emphasis is placed on indirect detection of 13C and 2H, especially by HPLC-1H NMR coupling, to analyse the isotopomer pattern of compounds in low concentration. The examples discussed in this paper were obtained from studies with Anigozanthos preissii (root cultures) (Haemodoraceae) and Eruca sativa (Brassicaceae).

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Authors and Affiliations

  1. Beutenberg Campus, Max-Planck-Institute for Chemical Ecology, Hans-Knöll-Str. 8, 07745, Jena, Germany

    Bernd Schneider, Jonathan Gershenzon, Gerson Graser, Dirk Hölscher & Bettina Schmitt

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  1. Bernd Schneider
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  2. Jonathan Gershenzon
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  3. Gerson Graser
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  4. Dirk Hölscher
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  5. Bettina Schmitt
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Correspondence to Bernd Schneider.

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Schneider, B., Gershenzon, J., Graser, G. et al. One-dimensional 13C NMR and HPLC-1H NMR techniques for observing carbon-13 and deuterium labelling in biosynthetic studies. Phytochemistry Reviews 2, 31–43 (2003). https://doi.org/10.1023/B:PHYT.0000004196.73829.4e

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  • DOI: https://doi.org/10.1023/B:PHYT.0000004196.73829.4e

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