Abstract
It is found that the interaction of retinol, ergocalciferol, and cholecalciferol with electrochemically generated bromine proceeds rapidly and quantitatively. The stoichiometric coefficients of the reaction are 1 : 2, 1 : 7, and 1 : 3, respectively. A coulometric determination of microgram amounts of individual liposoluble antioxidants in model solutions was performed with RSD = 1–5%. The voltammetric response of retinol and α-tocopherol was studied at a stationary platinum microelectrode in 0.1 M HClO4 and 0.1 M CH3COONa in acetonitrile. The quantification limit for α-tocopherol is 2.7 × 10–4 M in 0.1 M HClO4, and the quantification limits for retinol are 4.1 × 10–5 M in 0.1 M HClO4 and 2.1 × 10–5 M in 0.1 M CH3COONa. A procedure for the coulometric determination of total free liposoluble antioxidants in human blood serum is proposed.
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REFERENCES
Gueguen, S., Herbeth, B., Siest, G., and Leroy, P., J. Chromatogr. Sci., 2002, vol. 40, no. 2, p. 69.
Pichini, S., Zuccaro, P., Pellegrini, M., et al., J. Liq. Chromatogr. Relat. Technol. , 2002, vol. 25, no. 5, p. 781.
Jung, B.H., Chang, B.S., and Chung, B.C., Anal. Lett., 2001, vol. 34, no. 13, p. 2303.
Ha, S. and Ta, Y., Spectrosc. Spectr. Anal., 2002, vol. 22, no. 4, p. 693.
Rousseau, G. and Varin, F., J. Chromatogr. Sci., 1998, vol. 36, no. 5, p. 247.
Schwihhelova, Z. and Wilhelm, J., Chem. Zvesti, 1998, no. 52, p. 538.
Boyer, F.O., Romero, J.M.F., Decastro, M.D.L., and Quesada, J.M., Analyst (Cambridge, U.K.), 1999, vol. 124, no. 3, p. 401.
Van Pelt, C.K., Huggarty, P., and Brenna, J.Th., Anal. Chem., 1998, vol. 70, no. 20, p. 4369.
Tirmenstein, M.A., Watson, B.W., Haar, N.C., and Farris, M.W., J. Chromatogr., B, 1998, vol. 707, nos. 1-2, p. 308.
Dueker, S.R., Mercer, R.S., Jones, A.D., and Clifford, A.J., Anal. Chem., 1998, vol. 70, no. 7, p. 1369.
Abdullin, I.F., Turova, E.N., Ziyatdinova, G.K., and Budnikov, G.K., Zh. Anal. Khim., 2002, vol. 57, no. 8, p. 864.
Nadirov, N.K., Tokoferoly (vitaminy gruppy E)-biologicheski aktivnye veshchestva (Tocopherols (E-group Vitamins) as Biologically Active Substances), Moscow: Znanie, 1981.
Elektrokhimiya organicheskikh soedinenii (The Electrochemistry of Organic Compounds), Tomilov, A.P. and Feoktistov, L.G., Eds., Moscow: Mir, 1976.
Berezovskii, V.M., Khimiya vitaminov (The Chemistry of Vitamins), Moscow: Pishchevaya Promyshlennost', 1973.
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Budnikov, G.K., Ziyatdinova, G.K. & Gil'metdinova, D.M. Determination of Some Liposoluble Antioxidants by Coulometry and Voltammetry. Journal of Analytical Chemistry 59, 654–658 (2004). https://doi.org/10.1023/B:JANC.0000035278.20459.9e
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DOI: https://doi.org/10.1023/B:JANC.0000035278.20459.9e