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An improved chromosome preparation from male gametophyte of Laminaria japonica (Heterokontophyta)

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Abstract

An improved method for the preparation of chromosomes from the male gametophyte of the alga Laminaria japonica Aresch. was described. The male gametophyte was pretreated with pDB (p-dichlorobenzene) and 8-hydroxyquinoline in order to clear cell wall and soften cytoplasm. The samples were treated by mordant iron alum [FeNH4 (SO4)2⋅6H2O] followed by staining with haemotoxylin. Well-spread and highly stained chromosomes were observed without precipitation. The chromosome number of male gametophyte of L. japonica was estimated to be 31. %

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Author notes

  1. Li Ran Zhou

    Present address: Department of Biology, The Chinese University of Hong Kong, Shatin, N.T. Hong Kong

Authors and Affiliations

  1. Department of Marine Biotechnology, College of Marine Life Sciences, Ocean University of Qingdao, Qingdao, China

    Li Ran Zhou, Ji Xun Dai & Song Dong Shen

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  1. Li Ran Zhou
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  2. Ji Xun Dai
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  3. Song Dong Shen
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Ran Zhou, L., Xun Dai, J. & Dong Shen, S. An improved chromosome preparation from male gametophyte of Laminaria japonica (Heterokontophyta). Hydrobiologia 512, 141–144 (2004). https://doi.org/10.1023/B:HYDR.0000020319.36532.eb

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  • DOI: https://doi.org/10.1023/B:HYDR.0000020319.36532.eb

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