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Inhibitory effect of polypeptide from Chlamys farreri on UVA-induced apoptosis in human keratinocytes

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Abstract

Polypeptide from Chlamys farreri (PCF, M r = 879) is a novel marine active product isolated from gonochoric Chinese scallop Chlamys farreri which has been served as sea food for several thousand years. As an octapeptide, PCF consists of 8 amino acids, namely, Pro, Asn, Ser, Thr, Arg, Hyl, Cys, and Gly. PCF had been identified as a marine chemopreventive drug that protected hairless mice's epidermis against UV-induced damage in our previous study. However, the molecular mechanisms that underlie the effect of PCF on ultraviolet A-induced apoptosis in ketatinocytes are not well understood yet. In the present study, PCF was investigated as a potential inhibitory agent for UVA-induced apoptosis in a human keratinocyte cell line, HaCaT. The effects of PCF on UVA-induced generation of ROS and MDA, DNA damage, apoptosis rate were examined. We also investigated whether PCF could inhibit UVA-induced decreasing of mitochondrial membrane potential and the changing of morphology of the cells. We found that, compared with UVA only group, PCF attenuated UVA-induced generation of ROS and MDA, increased the mitochondrial membrane potential, and decreased the apoptosis rate. These results indicate that PCF may protect HaCaT keratinocytes against UVA-induced apoptosis.

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Authors and Affiliations

  1. Department of Pharmacology, Medical College of Qingdao University, Qingdao, China

    Mei Dou, XiangGuo Chen & ChunBo Wang

  2. Department of Pathophysiology, Medical College of Qingdao University, Qingdao, China

    YanTao Han

  3. Affiliated Hospital of Medical College of Qingdao University, Qingdao, China

    ZhiWu Han

  4. Yellow Sea Fisheries Research Institute, Qingdao, China

    YueJun Wang

  5. Laboratory Center, Medical College of Qingdao University, Qingdao, China

    JinShan Tan & YingYi Hou

Authors
  1. Mei Dou
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  2. YanTao Han
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  3. ZhiWu Han
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  4. XiangGuo Chen
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  7. ChunBo Wang
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Dou, M., Han, Y., Han, Z. et al. Inhibitory effect of polypeptide from Chlamys farreri on UVA-induced apoptosis in human keratinocytes. Invest New Drugs 22, 391–398 (2004). https://doi.org/10.1023/B:DRUG.0000036681.18225.32

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  • DOI: https://doi.org/10.1023/B:DRUG.0000036681.18225.32

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