Abstract
Acetic acid is by-product from fermentation processes for producing succinic acid using Mannheimia succiniciproducens. To obtain pure succinic acid from the final fermentation broth, acetic acid was selectively removed based on the different extractability of succinic acid and acetic acid with pH using tri-n-octylamine (TOA) as extractant. When successive batch extractions were performed using 0.25 mol TOA kg−1 dissolved in 1-octanol at pH 5, the mol ratio of succinic acid to acetic acid before extraction was 4.9 and the final ratio after the fourth batch was 9.4.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Canari R, Eyal AM (2003) Selectivity in monocarboxylic acids extraction from their mixture solutions using an amine-based extractant: effect of pH. Ind. Eng. Chem. Res. 42: 1301–1307.
Eyal AM, Canari R (1995) pH dependence of carboxylic and mineral acid extraction by amine-based extractants: effect of pKa, amine basicity, and diluent properties. Ind. Eng. Chem. Res. 34: 1789–1798.
Hong YK, Hong WH (2000) Extraction of succinic acid with 1-octanol/n-heptane of mixed tertiary amine. Biopro. Eng. 23: 535–538.
Hong YK, Hong WH, Chang HN (2000) Selective extraction of succinic acid from binary mixture of succinic acid and acetic acid. Biotechnol. Lett. 22: 871–874.
Jain MK, Datta R, Zeikus JG (1989) High-value organic acids fermentation-emerging processes and products. In: Ghosh TK, eds. Bioprocess Engineering the First Generation. Chichester: Ellis Harwood Press, pp. 366–398.
Kirsch T, Maurer G (1997) Distribution of binary mixtures of citric, acetic and oxalic acids between water and organic solutions of tri-n-octylamine. Part I. Organic solvent toluene. Fluid Phase Equilib. 131: 213–231.
Lee PC, Lee SY, Hong SH, Chang HN (2002) Isolation and characterization of a new succinic acid-producing bacterium, Man-nheimia succiniciproducens MBEL55E, from bovine rumen. Appl. Microbiol. Biotechnol. 58: 663–668.
Morales AF, Albet J, Kyuchoukov G, Malmary G, Molinier J (2003) Influence of extractant (TBP and TOA), diluent, and modifier on extraction equilibrium of monocarboxylic acids. J. Chem. Eng. Data, 48: 874–886.
San-Martin M, Pazos C, Coca J (1992) Reactive extraction of lactic acid with alamine 336 in the presence of salts and lactose. J. Chem. Technol. Biotechnol. 54: 1–6.
Tamada JA, Kertes AS, King CJ (1990) Extraction of carboxylic acids with amine extractants. 1. Equilibria and law of mass action modelling. Ind. Eng. Chem. Res. 29: 1319–1326.
Tung LA, King CJ (1994) Sorption and extraction of lactic and succinic acids at pH >pKa1. I. factors governing equilibria. Ind. Eng. Chem. Res. 33: 3217–3223.
Yang ST, White SA, Hsu ST (1991) Extraction of carboxylic acids with tertiary and quaternary amines: effect of pH. Ind. Eng. Chem. Res. 30: 1335–1342.
Zeikus JG, Jain MK, Elankovan P (1999) Biotechnology of succinic acid production and markets for derived industrial products. Appl. Microbiol. Biotechnol. 51: 545–552.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Huh, Y.S., Hong, Y.K., Hong, W.H. et al. Selective extraction of acetic acid from the fermentation broth produced by Mannheimia succiniciproducens . Biotechnology Letters 26, 1581–1584 (2004). https://doi.org/10.1023/B:BILE.0000045656.00138.93
Issue Date:
DOI: https://doi.org/10.1023/B:BILE.0000045656.00138.93