Abstract
In this study, a monoclonal antibody to the terminal oxidase of the alternative pathway from Sauromatum guttatum was used to detect the expression of alternative oxidase (AOX) protein in tomato mitochondria. The results show that there was an obvious correlation between the ethylene-induced apoptosis and the levels of AOX protein in tomato cells undergoing ethylene-induced apoptosis. In addition, when tomato protoplasts were preincubated with 2 mM salicylhydroxamic acid (SHAM), an inhibitor of the alternative pathway, before their exposure to ethylene, the TUNEL positive reaction and DNA fragmentation were obviously accelerated. We suggest that AOX may play an important role in protecting tomato protoplasts against ethylene-induced apoptosis.
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Lei, XY., Zhu, RY., Zhang, GY. et al. Possible involvement of the mitochondrial alternative pathway in ethylene-induced apoptosis in tomato protoplasts. Plant Growth Regulation 41, 111–116 (2003). https://doi.org/10.1023/A:1027355502538
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DOI: https://doi.org/10.1023/A:1027355502538