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High performance separation technologies and spectroscopic tools for plant extract characterization in phytomics

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Abstract

The review is concerned with fast analytical methods for the qualitative and quantitative determination of plant constituents and phytopharmaceutical products. Emphasis is put on the determination of leading compounds, the role of the stationary phase in the analysis of sugars, phenolic compounds, proteins or DNA-mutations and the use of non-standard analytical tools such as micro high performance liquid chromatography (μ-LC) or near infrared reflectance spectroscopy (NIR) next to conventional high performance liquid chromatography (HPLC). Finally the importance of spectroscopic methods such as mass spectrometry (MS) in phytomics will be discussed giving examples for HPLC-MS and μ-HPLC-MS hyphenation.

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References

  • Bakry R, Stöggl WM, Hochleitner EO, Stecher G, Huck CW & Bonn GK (2003) PS/DVB embedded silica stationary phases for liquid chromatography. Anal Chem in prep.

  • Bonn GK, Huber C & Oefner P (1996) Nucleic Acid Separation on Alkylated non porous Polymer Beads. U.S. Patent Nr. 5, 585, 236.

  • Boughtflower RJ, Underwood T & Paterson CJ (1995) Capillary Electrochromatography-Some Important Considerations in the Preparation of Packed Capillaries and the Choice of Mobile Phase Buffers. Chromatographia 40: 329–335.

    Article  CAS  Google Scholar 

  • Corradini C, Corradini D, Huber CG & Bonn GK (1994) Synthesis of a polymeric-based stationary phase for carbohydrate separation by high-pH anion-exchange chromatography with pulsed amperometric detection. J. Chromatogr A 685: 213.

    Article  CAS  Google Scholar 

  • Corradini C, Corradini D & Huber CG, Bonn GK (1995) High-performance anion-exchange chromatography of carbohydrates using a new resin and pulsed amperometric detection. Chromatographia 41: 511.

    Article  CAS  Google Scholar 

  • El Rassi (ed.) (2001) Carbohydrate Analysis by Modern Chromatography and Electrophoresis. Elsevier Science B.V.

  • Fauconneau B, Waffo-Teguo P, Huguet F, Barrier L, Decendit A & Merillon JM (1997) Comparative study of radical scavenger and antioxidant properties of phenolic compounds from Vitis vinifera cell cultures using in vitro tests. Life Sci. 61: 2103.

    Article  PubMed  CAS  Google Scholar 

  • Grummisch U (1998) Qualitative and quantitative near infrared spectroscopy applications in the pharmaceutical industry. Pharm. Ind. 60: 1002–1006.

    CAS  Google Scholar 

  • Hjertén S, Liao JL & Zhang R (1989) High-performance liquid chromatography on continuous polymer beds. J. Chromatogr A 473: 273–275.

    Article  Google Scholar 

  • Hochleitner EO, Bakry R, Huck CW, Flores F, Stöggl WM, Stecher G & Bonn GK (2003) Analysis of Isolectins on Non-Porous Particles and Monolithic Polystyrene-Divinylbenzene based Sorbents and Electrospray Ionisation Mass Spectrometry. Int. J. Mass Spectrom. 223–224: 519–526.

    Google Scholar 

  • Hostettmann K, Hostettmann M & Marston A (1998) Preparative Chromatography Techniques-Applications in Natural Product Isolation. Springer, Berlin.

    Google Scholar 

  • Huber CG, Oefner PJ & Bonn GK (1993a) High-resolution liquid chromatography of oligonucleotides on highly crosslinked poly(styrene-divinylbenzene) particles. Anal. Biochem. 212: 351–358.

    Article  PubMed  CAS  Google Scholar 

  • Huber CG, Oefner PJ & Bonn GK (1993b) Rapid analysis of biopolymers on modified non-porous poly-styrene/divinylbenzene particles. Chromatographia 37: 653–658.

    Article  CAS  Google Scholar 

  • Huber CG, Oefner PJ, Preuß E & Bonn GK (1993c) High-resolution liquid chromatography of DNA fragments on non porous poly(styrene-divinylbenzene) particles. Nucl. Acids Res. 21: 1061–1066.

    PubMed  CAS  Google Scholar 

  • Huck CW (2002) Near Infrared Spectrosc., Proceedings of the 10th Int. Conf. Verona, Italy, 1999, 491.

  • Huck CW, Huber CG, Lagoja IM, Ongania K-H, Scherz H & Bonn GK (1999a) Isolation and Structural Elucidation of 3′,4′,5′-Trimethoxyflavone from the Flowers of Primula veris. Planta Med. 65: 491.

    Article  CAS  PubMed  Google Scholar 

  • Huck CW, Maurer R, Popp M, Basener N & Bonn GK (1999b) Quantitative fourier transform near infrared reflectance spectroscopy (NIRS) compared to high performance liquid chromatography (HPLC) of a flavone in Primulae veris Flos extracts. Pharm. Pharmacol. Lett. 9: 26–29.

    CAS  Google Scholar 

  • Huck CW, Huber CG, Ongania K-H & Bonn GK (2000a) Isolation and Characterization of Methoxylated Flavones in the Flowers of Primula veris by Liquid Chromatography and Mass Spectrometry. J. Chromatogr. A 870: 453–462.

    Article  PubMed  CAS  Google Scholar 

  • Huck CW & Bonn GK (2000b) Review: Polymer based sorbents for solid-phase extraction. J. Chromatogr. A 885: 51–72.

    Article  PubMed  CAS  Google Scholar 

  • Huck CW, Maurer R & Bonn GK (2000c) Near Infrared Spectrosc., Proceedings of the 9th Int. Conf. Kyongju, Korea, 1999, 487–491.

  • Jochum M, Bakry R, Wartusch I, Huck CW, Engelhardt H & Bonn GK (2002) Analysis of Carbohydrates Using Different Quaternized Polystyrene-Divinylbenzene Particles and Pulsed Amperometric Detection. Chromatographia 56: 263–268.

    Google Scholar 

  • Khandurina J & Guttman A (2002) Bioanalysis in microfluidic devices. J. Chromatogr. A 943: 159–183.

    Article  PubMed  CAS  Google Scholar 

  • Lüderwald I, Müller M, Günzler H, Borsdorf R, Danzer K, Fresenius W, Huber W, Lüderwald ITG & Wisser HE (1993) Analytiker Taschenbuch 11. Springer, Berlin.

    Google Scholar 

  • Martens H & Naes T (1994) Multivariate Calibration. John Wiley & Sons Inc., Chicester.

    Google Scholar 

  • Montanarella L, Bassani MR & Breas O (1995) Chemometric classification of some European wines using pyrolysis mass spectrometry. Rapid Commun. Mass Spectrom. 9(15): 1589–1593.

    Article  CAS  Google Scholar 

  • Neue UD (1997) HPLC Columns-Theory, Technology and Practice. Wiley, New York.

    Google Scholar 

  • Niessen WMA (1999) Liquid Chromatography-Mass Spectrometry. Marcel Dekker Inc. New York.

    Google Scholar 

  • Oefner P & Underhill PA (1995) Comparative DNA sequencing by denaturing high-performance liquid chromatography (DHPLC). Am. J. Hum. Genet.57 (Suppl.) A266.

    Google Scholar 

  • Oefner PJ & Underhill PA (1998) Detection of Nucleic Acid Heteroduplex Molecules by Denaturing High-Performance Liquid Chromatography and Methods for Comparative Sequencing. U.S. Patent 5,795,976.

  • Pirker R, Huck CW & Bonn GK (2002) Simultaneous determination of hypericin and hyperforin in human plasma and serum using liquid-liquid extraction, high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry. J. Chromatogr. B 777 (1–2), 147–153.

    Article  CAS  Google Scholar 

  • Popp M, Bonn GK, Huck CW & Guggenbichler W (2002) NIR spectroscopic method for classifying wine and coffee. PCT Int. Appl. 31 WO 0297431.

  • Rapp E & Bayer E (2000) Improved column preparation and performance in capillary electrochromatography. J. Chromatogr. A 887: 367–378.

    Article  PubMed  CAS  Google Scholar 

  • Renaud S & De Lorgeril M (1992) Wine, alcohol, platelets, and the French paradox for coronary heart disease. The Lancet 339: 1523.

    Article  CAS  Google Scholar 

  • Scherz H & Bonn GK (1998) Analytical Chemistry of Carbohydrates. Thieme Stuttgart, New York.

    Google Scholar 

  • Sivertsen HK, Holen B, Nicolaysen F & Risvik E (1999) Classification French red wines according to their geographical origin by the use of multivariate analyses. J. Sci. Food Agric. 79: 107.

    Article  CAS  Google Scholar 

  • Skoog DA & Leary JJ (1996) Instrumentelle Analytik. Springer, Berlin.

    Google Scholar 

  • Stecher G, Huck CW, Popp M & Bonn GK (2001) Determination of flavonoids and stilbenes in red wine and related biological products by HPLC and HPLC-ESI-MS-MS. Fresenius J. Anal. Chem. 371: 73–80.

    Article  PubMed  CAS  Google Scholar 

  • Stecher G, Huck CW, Stöggl WM & Bonn GK (2002) Phytoanalysis-A Challange in Phytomics. Trends Analyt. Chem. 22: 1–14.

    Article  CAS  Google Scholar 

  • Stöggl WM, Huck CW, Scherz H, Popp M & Bonn GK (2001) Analysis of Vitamin E in Food and Phytopharmaceutical Preparation by HPLC and HPLC-MS-MS. Chromatographia 54: 179–185.

    Google Scholar 

  • Van Wijk KJ (2001) Challenges and Prospects of Plant Proteomics. Plant Physiol. 126: 501–508.

    Article  PubMed  CAS  Google Scholar 

  • Wagner H & Kraus S (2000) News on immunologically active plant polysaccharides. Proc. Phytochem. Soc. Euro. 44: 1–14.

    CAS  Google Scholar 

  • Weiss J (2001) Ionenchromatographie. Wiley VCH, Weinheim, Berlin.

    Google Scholar 

  • Worm N (1998) Täglich Wein. Hallwag AG, Bern.

    Google Scholar 

  • Xie B, Ramanathan K & Daielsson B (2000) Mini/micro thermal biosensors and other related devices for biochemical/clinical analysis and monitoring. TRAC 19: 340–349.

    CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University Innsbruck, Innrain 52a, 6020-, Innsbruck, Austria

    G. Stecher, C.W. Huck, W.M. Stöggl, W. Guggenbichler, R. Bakry & G.K. Bonn

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  1. G. Stecher
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  2. C.W. Huck
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  3. W.M. Stöggl
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  4. W. Guggenbichler
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  5. R. Bakry
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  6. G.K. Bonn
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Stecher, G., Huck, C., Stöggl, W. et al. High performance separation technologies and spectroscopic tools for plant extract characterization in phytomics. Phytochemistry Reviews 1, 413–426 (2002). https://doi.org/10.1023/A:1026083122460

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