Abstract
Purpose : Tripronucleate (3pn) development after conventional insemination (CONV) or ICSI was analyzed to estimate the rate of second polar body retention giving rise to 3pn formation.
Methods : Data from 453 consecutive IVF cycles were reviewed during a 6-month period. Mature oocytes were monitored in ICSI (n = 3195) and CONV (n = 2274) groups by fertilization assessment 16–18 h post-insemination. Ovulation induction protocols and in vitro culture conditions remained constant during the study interval.
Results : Normal (2pn) fertilization occurred in 74.2% and 70.5% for CONV and ICSI groups, respectively (p < 0.003). 1pn formation was observed in 4.5% of CONV oocytes, and 2.5% of ICSI oocytes (p < 0.001); 3pn formation was 8.1% in the CONV group, and 2.5% in the ICSI group (p < 0.0001). We observed 4pn formation in 0.4% of oocytes in the CONV group, but in only 0.04% of oocytes fertilized with ICSI (p < 0.007). Cellular degeneration occurred in 2.4% of oocytes inseminated conventionally, and in 3.5% of oocytes fertilized by ICSI (p = 0.02). Maternal age did not impact pronuclear status.
Conclusions : We found the 3pn formation rate after ICSI to be approximately one-third that observed in the CONV group. Extrapolating the ICSI data to the CONV data, it may be inferred that 2.5% of 3pn development after CONV was due to second polar body retention. This suggests that 5.6% of CONV oocytes showed dispermic fertilization. Decreasing oocyte quality with increasing maternal age had no apparent influence on any of the fertilization outcomes.
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References
Staessen C, Van Steirteghem A: The chromosomal constitution of embryos developing from abnormally fertilized oocytes after intracytoplasmic sperm injection and conventional in-vitro fertilization. Hum Reprod 1997;12:321-327
Spandorfer SD, Avrech OM, Colombero LT, Palermo GD, Rosenwaks Z: Effect of parental age on fertilization and pregnancy characteristics in couples treated by intracytoplasmic sperm injection. Hum Reprod 1998;13:334-338
Veeck LL (ed): An Atlas of Human Gametes and Conceptuses: An Illustrated Reference for Assisted Reproductive Technology. New York, Parthenon Publishing Group, 1999
Balakier H: Tripronucleate human zygotes: The first cell cycle and subsequent development. Hum Reprod 1993;8:1892-1897
Munné S, Tang YX, Grifo J, Cohen J. Origin of single pronucleated human zygotes. J Assist Reprod Genet 1993;10:276-279
Palermo GD, Munné S, Colombero LT, Cohen J, Rosenwaks Z: Genetics of abnormal human fertilization. Hum Reprod 1995;10(suppl 1):120-127
Sultan KM, Munné S, Palermo GD, Alikani M, Cohen J: Chromosomal status of uni-pronuclear human zygotes following in-vitro fertilization and intracytoplasmic sperm injection. Hum Reprod 1995;10:132-136
Kola I, Trounson A, Dawson G, Roger P: Tripronucleate human oocytes: Altered cleavage patterns and subsequent karyotypic analysis of embryos. Biol Reprod 1987;37:395-401
Balakier H, Squire J, Casper RF: Characterization of abnormal one pronuclear human oocytes by morphology, cytogenics and in-situ hybridization. Hum Reprod 1993;8:402-408
Macas E, Imthurn B, Roselli M, Keller PJ: Chromosome analysis of single-and multipronucleated human zygotes produced after the intracytoplasmic sperm injection procedure. J Assist Reprod Genet 1996;13: 345-350
Grossmann M, Calafell JM, Brandy N, Vanrell JA, Rubio C, Pellicer A, Egozcue J, Santalo J: Origin of tripronucleate zygotes after intracytoplasmic sperm injection. Hum Reprod 1997;12:2762-2765
Plachot M, Crozet N: Fertilization abnormalities in human in-vitro fertilization. Hum Reprod 1992;7(suppl 1):89-94
Palermo GD, Alikani M, Bertoli M, Colombero LT, Moy F, Cohen J, Rosenwaks Z: Oolemma characteristics in relation to survival and fertilization patterns of oocytes treated by intracytoplasmic sperm injection. Hum Reprod 1996;11:172-176
Rosenbusch B, Schneider M, Glaser B, Brucker C. Cytogenetic analysis of giant oocytes and zygotes to asses their relevance for the development of digynic triploidy. Hum Reprod 2002;17:2388-2393
Palermo G, Munné S, Cohen J: The human zygote inherits its mitotic potential from the male gamete. Hum Reprod 1994;9:1220-1225
Gras L, Trounson AO: Pregnancy and birth resulting from transfer of a blastocyst observed to have one pronucleus at the time of examination for fertilization. Hum Reprod 1999;14:1869-1871
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Porter, R., Han, T., Tucker, M.J. et al. Estimation of Second Polar Body Retention Rate After Conventional Insemination and Intracytoplasmic Sperm Injection: In Vitro Observations from More Than 5000 Human Oocytes. J Assist Reprod Genet 20, 371–376 (2003). https://doi.org/10.1023/A:1025481011680
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DOI: https://doi.org/10.1023/A:1025481011680