Abstract
An efficient protocol has been developed for rapid mass propagation of Tylophora indica from leaf derived callus. Optimal callus was developed from leaf explants on Murashige and Skoog (MS) basal medium supplemented with 10 μ 2,4,5-T. Adventitious shoots were regenerated (85%) from the surface of the callus on MS medium supplemented with 5 μM Kinetin. Individual elongated shoots were rooted on half-strength MS medium containing 0.5 μM IBA. Regenerated plantlets with well developed shoots and roots were successfully transferred to soil. The study demonstrated a dedifferentiated callogenic propagation route via adventitious shoot development in T. indica, which could be useful for large scale multiplication of this endangered medicinal plant.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ahroni A, Zuker A, Rojen Y, Shejtman H & Vainstein (1997) An efficient method of adventitious shoot regeneration from stem explants of Gypsophila. Plant Cell Tiss. Org. Cult. 49: 101–106
Anonymous (1976) The wealth of India. Raw Materials, Vol X. Publications and Information Directorate, CSIR, New Delhi, India
Benzamine BD, Heble MR & Chadha MS (1979) Alkaloid synthesis in tissue culture regenerated plants of Tylophora indica Merr. (Asclepiadeceae). Z. Pflanzenphysiol. 92: 77–84
Fracro F & Echeverrigaray S (2001) Micropropagaton of Cunila galoides, a popular medicinal plant of South Brazil. Plant Cell Tiss. Org. Cult. 64: 1–4
Jusaitis M (1997) Micropropagation of adult Swainsona formosa Leguminosae: Papilionoideae: Galegeae.In vitro Cell Dev. Biol. Plant 33: 213–220
Luis PBC, Adraine CMGM, Silvica BRCC & Anna Christina MB (1999) Plant regeneration from seedling explants of Eucalyptus grandisXE. urophylla. Plant Cell Tiss. Org. Cult. 56: 17–23
Lusia MAV & Rojas G (1996) In vitro propagation of Mimosa teniflora (Willd.) Poiret, a Mexican medicinal tree. Plant Cell Rep. 16: 80–82
Mhatre M, Bapat VA & Rao PS (1984) Plant regeneration in protoplast cultures of Tylophora indica. J. Plant Physiol. 115: 231–235
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant. 15: 473–497
Prakash E, Sha Valli Khan PS, Sairam Reddy P & Rao KR (1999) Regeneration of plants from seed-derived callus of Hybanthus enneaspermus L. Muell., a rare ethnobotanical herb. Plant Cell Rep. 18: 873–878
Rao PS & Narayanaswamy S (1972) Morphgenetic investigation in callus cultures of Tylophora indica. Physiol. Plant. 27: 271–276
Rao PS, Narayanaswamy S & Benzamine BD (1970) Differentia-tion of ex ovulo of embryos and plantlets in stem tissue cultures of Tylophora indica. Physiol. Plant. 23: 140–144
Reddy PS, Rodrigues R & Rajasekharan R (2001) Shoot or-ganogenesis and mass propagation of Coleus forskholii from leaf derived callus. Plant Cell Tiss. Org. Cult. 66: 183–188
Sarasan V, Sonia EV & Nair GM (1994) Regeneration of Indian Sarasparilla, Hemiesmus indicus R. Br. Indian J. Exp. Biol. 32: 284–287
Sharma N & Chandel KPS (1992) Effect of ascorbic acid on axillary shoot induction in Tylophora indica (Burm. f.) Merrill. Plant Cell Tiss. Org. Cult. 29: 109–113
Sharma N, Chandel KPS & Srivastava VK (1991) In vitro propagation of Coleus forskholii Briq., a threatened medicinal plant. Plant Cell Rep. 10: 67–70
Wldi E, Schaffner W & Berger KB (1998) In vitro propagation of Petasites hybridus (Asteraceae) from leaf and petiole explants and from inflorescence buds. Plant Cell Rep. 18: 336–340
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Faisal, M., Anis, M. Rapid mass propagation of Tylophora indica Merrill via leaf callus culture. Plant Cell, Tissue and Organ Culture 75, 125–129 (2003). https://doi.org/10.1023/A:1025084203958
Issue Date:
DOI: https://doi.org/10.1023/A:1025084203958