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Molecular cloning and characterization of rKAB1, which interacts with KARP-1, localizes in the nucleus and protects cells against oxidative death

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Abstract

The Ku autoantigen/KARP-1 (Ku86 autoantigen related protein-1) plays an important role in the double-strand break repair of mammalian DNA as a DNA-binding component of DNA-dependent protein kinase (DNA-PK) complex. KARP-1 is differently transcribed from the human Ku86 autoantigen gene locus and it is implicated in the control of DNA-dependent protein kinase activity. We cloned rKAB1, a rat homolog of KAB1 (KARP-1 binding protein 1 of human) from a rat hippocampal cDNA library. rKAB1 mRNA was specifically expressed in the brain and the thymus. EGFP-tagged rKAB1 protein localized in cell nucleus and in the condensed chromosome during the mitotic cell division. We found that rKAB1 works as a protective protein against cell damage by oxidative stress.

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Authors and Affiliations

  1. Department of Pharmacology, Osaka University Medical School, 2-2 Yamada-oka, Suita, Osaka, 565-0871, Japan

    Eunju Do, Eiichi Taira, Yasuyuki Irie, Yehua Gan, Hidekazu Tanaka, Che-Hui Kuo & Naomasa Miki

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  1. Eunju Do
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  2. Eiichi Taira
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  3. Yasuyuki Irie
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  4. Yehua Gan
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  5. Hidekazu Tanaka
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  6. Che-Hui Kuo
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  7. Naomasa Miki
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Do, E., Taira, E., Irie, Y. et al. Molecular cloning and characterization of rKAB1, which interacts with KARP-1, localizes in the nucleus and protects cells against oxidative death. Mol Cell Biochem 248, 77–83 (2003). https://doi.org/10.1023/A:1024157515342

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