Abstract
A 2D-HSQC-carbon selective/proton selective-constant time COSY, 2D-HSQC-(sel C, sel H)-CT COSY experiment, which is applicable to uniformly 13C isotopically enriched samples (U-13C) of oligosaccharides or oligonucleotides is proposed for the measurement of proton–proton RDC in crowded regions of 2D-spectra. In addition, a heteronuclear constant time-COSY experiment, 13C-13C CT-COSY, is proposed for the measurement of one bond carbon–carbon RDC in these molecules. These two methods provide an extension, to U-13C molecules, of the original homonuclear constant time-COSY experiment proposed by Tian et al. (1999) for saccharides. The combination of a number of these RDC with NOE data may provide the method of choice to study oligosaccharide conformation in the free and receptor-bound state.
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Martin-Pastor, M., Canales-Mayordomo, A. & Jiménez-Barbero, J. NMR experiments for the measurement of proton–proton and carbon–carbon residual dipolar couplings in uniformly labelled oligosaccharides. J Biomol NMR 26, 345–353 (2003). https://doi.org/10.1023/A:1024096807537
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DOI: https://doi.org/10.1023/A:1024096807537