Abstract
Two inhibitors of metabolite translocators, sodium pyrophosphate (NaPP) and D,L-glyceraldehyde (DLG), respectively, were added into suspension cultures of Taxus chinensis var. mairei at the early and late growth phases to investigate the translocation of isopentenyl pyrophosphate (IPP) from cytoplasm to plastids for Taxol biosynthesis. NaPP and DLG hardly affected cell biomass and viability regardless of the phases of their introduction. The Taxol content varied less when NaPP or DLG was added at day 7 but decreased obviously when NaPP or DLG was introduced at day 14. It is thus inferred that NaPP and DLG inhibited Taxol biosynthesis by blocking IPP translocation at the late growth phase, suggesting that the translocation of IPP be involved only at the late growth phase of Taxus cells.
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References
Adam KP & Zapp J (1998) Biosynthesis of the isoprene units of chamomile sesquiterpenes. Phytochemistry 48: 953–959
Bäuerle R, Lütke-Brinkhaus F, Ortman B, Berger S & Kleinig H (1990) Prenyl lipid and fatty-acid synthesis in isolated Acetabularia chloroplasts. Planta 181: 229–233
Connolly JD & Hill RA (1992) Dictionary of Terpenoids. Chapman and Hall, New York
Edwards G & Walker D (1983) C3:C4:Mechanisms, and Cellular and Environmental Regulation of Photosynthesis (pp. 201–246). University of California Press, Berkeley, CA
Eisenhauer EA & Vermorken JB (1998) The Taxoid: comparative clinical pharmacology and therapeutic potential. Drugs 55: 5–30
Eisenreich W, Menhard B, Hylands PJ, Zenk MH & Bacher A (1996) Studies on the biosynthesis of taxol: the taxane carbon skeleton is not of mevalonoid origin. Proc. Natl. Acad. Sci. USA 93: 6431–6436
Eisenreich W, Rohdich F & Bacher A (2001) Deoxyxylulose phosphate pathway to terpenoids. Trends Plant Sci. 6: 78–84
Heintze A, Goerlach J, Leuschneer C, Hoppe P, Hagelstein P, Schulze-Siebert D & Schultz G (1990) Plastidic isoprenoid synthesis during chloroplast development: change from metabolic autonomy to division of labor stage. Plant Physiol. 93: 1121–1127
Iborra JL, Guardiola J, Montaner S, Canovas M & Manjon A (1992) 2,3,5-Triphenyltetrazolium chloride as a viability assay for immobilized plant cells. Biotechnol. Tech. 6: 319–322
Knöess W, Reuter B & Zapp J (1997) Biosynthesis of the labdane diterpene marrubiin in Marrubium vulgare via a non-mevalonate pathway. Biochem. J. 326: 449–454
McCaskill D & Croteau R (1995) Monoterpene and sesquiterpene biosynthesis in glandular trichomes of peppermint (Mentha× piperita) rely exclusively on plastid-derived isopentenyl diphosphate. Planta 197: 49–56
McCaskill D & Croteau R (1998) Some caveats for bioengineering terpenoid metabolism in plants. Trends Biotechnol. 16: 349–355
McGarvey DJ & Croteau R (1995) Terpenoid metabolism. Plant Cell 7: 1015–1026
Musser DA & Oseroff AR (1994) The use of tetrazolium salts to determine sites of damage to the mitochondrial electron transport chain in intact cells following in vitro photodynamic therapy with photofin II. Photochem. Photobiol. 59: 621–626
Phisalaphong M & Linden JC (1999) Kinetic studies of Paclitaxel Production by Taxus canadensis cultures in batch and semicontinuous with total cell recycle. Biotechnol. Prog. 15: 1072–1077
Ruklisha M & Paegle L (2001) Metabolic fluxes and L-lysine synthesis by Corynebacterium glutamicum in relation to cellular total reducing activity. Proc. Biochem. 36: 1233–1240
Soler E, Clastre M, Bantignies B, Marigo G & Ambid C (1993) Uptake of isopentenyl diphosphate by plastids isolated from Vitis vinifera (L.) cell suspensions. Planta 191: 324–329
Srinivasan V, Ciddi V, Bringi V & Shuler ML (1996) Metabolic inhibitors, elicitors, and precursors as tools for probing yield limitation in Taxane Production by Taxus chinensis. Cell Cult. Biotechnol. Prog. 12: 457–465
Stokes DM & Walker DA (1972) Photosynthesis by isolated chloroplasts. Biochem. J. 128: 1147–1157
Totté N, Charon L, Rohmer M, Compernolle F, Baboeuf I & Geuns JMC (2000) Biosynthesis of the diterpenoid steviol, an entkaurene derivative from Stevia rebaudiana Bertoni, via the methylerythritol phosphate pathway. Tetrahedron Lett. 41: 6407–6410
Zamir LO, Nedea ME & Garneau FX (1992) Biosynthetic building blocks of Taxus canadensis taxanes. Tetrahedron Lett. 33: 5235–5236
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Wang, YD., Yuan, YJ. & Wu, JC. Translocation of isopentenyl pyrophosphate for Taxol biosynthesis in suspension cultures of Taxus chinensis var. mairei . Plant Cell, Tissue and Organ Culture 74, 283–288 (2003). https://doi.org/10.1023/A:1024019809910
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DOI: https://doi.org/10.1023/A:1024019809910