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Identification of a sequence-tagged site (STS) marker linked to a restorer gene for Ogura cytoplasmic male sterility in radish (Raphanus sativus L.) by non-radioactive AFLP analysis

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Abstract

To identify DNA markers linked to a fertility restorer (Rf) genefor Ogura cytoplasmic male sterility in radish (Raphanus sativus L.),a non-radioactive, amplified fragment length polymorphism (AFLP) analysiswas performed on bulked DNA samples from male-sterile and male-fertileradishes. Ten male-fertile and 10 male-sterile plants selected arbitrarilyfrom an F2 population made by selfing of F1 plant from a crossbetween a male-sterile (`MS-Gensuke') plant and a restorer (`Comet') plantwere used as material. Using 32 AFLP primer pairs, one AFLP fragment(AFLP190) which is specific to the bulked DNA samples from male-fertileF2 plants was identified. AFLP190 was characterized by molecularcloning and nucleotide sequencing, and was converted to a sequence-taggedsite (STS) marker, STS190. A linkage analysis performed in 126individuals of two independent F2 populations showed tight linkageof STS190 to the Rf gene. The rate of recombination between themarker and Rf was estimated to be less than 1%, making STS1901.2 cM from the gene.

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Engineering, Kyoto Sangyo University, Motoyama, Kamigamo, Kita-ku, Kyoto, 603-8555, Japan

    Seiji Murayama, Toshiyuki Habuchi, Hiroshi Yamagishi & Toru Terachi

  2. Laboratory of Plant Genecology, Hokkaido National Agricultural Experiment Station, Hitsuji-ga-oka, Toyohira-ku, Sapporo, 062-8555, Japan

    Seiji Murayama

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  1. Seiji Murayama
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  2. Toshiyuki Habuchi
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  3. Hiroshi Yamagishi
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  4. Toru Terachi
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Murayama, S., Habuchi, T., Yamagishi, H. et al. Identification of a sequence-tagged site (STS) marker linked to a restorer gene for Ogura cytoplasmic male sterility in radish (Raphanus sativus L.) by non-radioactive AFLP analysis. Euphytica 129, 61–68 (2003). https://doi.org/10.1023/A:1021513331079

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  • DOI: https://doi.org/10.1023/A:1021513331079

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