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Photochemical transformations and bacterial utilization of high-molecular-weight dissolved organic carbon in a southern Louisiana tidal stream (Bayou Trepagnier)

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Abstract

UVirradiation of dissolved organic carbon (DOC) in the laboratory can producesmall, labile organic compounds utilizable by microbes, but few studies haveattempted to document this process in situ. 13Cnuclear magnetic resonance (NMR) was used to examine the bulk chemicalcomposition of natural and laboratory-irradiated high-molecular-weight DOC(HMW-DOC) from shaded (150 μmol m−2s−1 average light in surface water) and open (1500μmol m−2 s−1) field sitesoverone and a half years. 13C NMR revealed only small differences incarbon functional groups between laboratory irradiated and non-irradiatedHMW-DOC. However, bacterial protein productivity per cell (BPP) was enhanced innaturally irradiated samples of HMW-DOC in a field mesocosm experiment (p <0.05), suggesting that bacterial growth was enhanced by photochemicalproductionof labile DOC substrates. Absorbance characteristics such as spectral slope,absorbance at 350 nm, and the absorbance ratio 250nm/365 nm revealed that HMW-DOC was photoreactive,yetno differences in these values were found between samples irradiated with andwithout UV-B. In experiments conducted with simulated solar radiation in thelaboratory and with natural light in the field mesocosm experiment, UV-A(320–400 nm) and photosynthetically active radiation (PAR;400–700 nm) were more effective than UV-B (280–320nm) in HMW-DOC photolysis.

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Authors
  1. Erika Engelhaupt
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  2. Thomas S. Bianchi
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  3. Robert G. Wetzel
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  4. Matthew A. Tarr
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Engelhaupt, E., Bianchi, T.S., Wetzel, R.G. et al. Photochemical transformations and bacterial utilization of high-molecular-weight dissolved organic carbon in a southern Louisiana tidal stream (Bayou Trepagnier). Biogeochemistry 62, 39–58 (2003). https://doi.org/10.1023/A:1021176531598

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