Skip to main content
SpringerLink
Log in

On-line biomass monitoring in bench-scale stirred bioreactors using parts of a liquid chromatography system

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

A cell density monitor, comprising parts of a liquid chromatography system, was adapted for on-line real-time measurement of bacterial cell density up to 75 g l−1. The device dilutes the sample containing cells and measures the absorbancy at 280 nm. This detector gives an excellent correlation with off-line measurements, good reproducibility, a low limit of detection (3.5 mg l−1), and becomes a low cost alternative for fermentation monitoring in small stirred bioreactors.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Baxter PJ, Christian GD, Ruzicka J (1994) Rapid determination of total biomass from yeast fermentation using sequential injection. Analyst 119: 1807–1812.

    Google Scholar 

  • DeLisa MP, Li JC, Rao G, Weigand WA, Bentley WE (1999) Monitoring GFP-operon fusion protein expression during high cell density cultivation of Escherichia coli using an on-line optical sensor. Biotechnol. Bioeng. 65: 54–64.

    Google Scholar 

  • Dorschel CA, Ekmanis JL, Oberholtzer JE, Warren Jr FV, Bidlingmeyer BA (1989) LC detectors: evaluation and practical implications of linearity. Anal. Chem. 61: 951–968.

    Google Scholar 

  • Jung YH, Lee JD, Chang YK (1999) Development of a new device for on-line measurement of high cell concentrations. Biotechnol. Tech. 13: 459–462.

    Google Scholar 

  • Ripp J (1996) Analytical detection limit guidance. Department of Natural Resources, Wisconsin, USA.

    Google Scholar 

  • Sadar MJ (1998) Turbidity science: Technical Information Series, Booklet 11, 6th edn. Colorado: Hach Company. (Accessed July 2001, at URL www.hach.com/ASP/categories.asp)

  • Scragg AH (1991) Bioreactors in Biotechnology. Bristol: Ellis Horwood.

    Google Scholar 

  • Sonnleitner B (1996) New concepts for quantitative bioprocess research and development. Adv. Biochem. Eng./Biotechnol. 54: 155–188.

    Google Scholar 

  • Ziegler AC (2002) Issues related to use of turbidity measurements as a surrogate for suspended sediment. In: Turbidity and Other Sediment Surrogates Workshop, April 30–May 2, Reno, NV. Accessed June 2002, at URL http://ks.water.usgs.gov/Kansas/rtqw/

Download references

Author information

Authors and Affiliations

  1. Departamento de Química Biológica-CIQUIBIC, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina

    Diego O. Forcato, Rolando P. Pécora, Silvia C. Kivatinitz & Silvia C. Kivatinitz

Authors
  1. Diego O. Forcato
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rolando P. Pécora
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Silvia C. Kivatinitz
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Forcato, D.O., Pécora, R.P. & Kivatinitz, S.C. On-line biomass monitoring in bench-scale stirred bioreactors using parts of a liquid chromatography system. Biotechnology Letters 24, 1999–2003 (2002). https://doi.org/10.1023/A:1021110730741

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1021110730741

Search

Navigation