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Confinement stress and starvation modulate the induction of EROD activity after dietary exposure to PCB 126 in the Mozambique tilapia (Oreochromis mossambicus)

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Abstract

Polychlorinated biphenyls (PCBs) are environmental pollutants, accumulating in the food chain and inducing cytochrome P450 1A (CYP P450 1A) monooxygenase enzymes. This study aimed to investigate the influence of dietary PCB 126 exposure on cytochrome P4501A-associated enzyme activities in head kidney, liver, gill and intestine of unstressed and stressed tilapia (Oreochromis mossambicus) before and after starvation. Fish were fed diets containing 0 μg (control), 0.5 μg (low) or 50 μg (high) PCB126 per kg body weight per day for 7 days. After the PCB-exposure each treatment group was sampled directly, or was subsequently exposed to confinement stress. Replicate groups remained undisturbed, starved for 3 weeks and were thereafter sampled directly or after confinement. Catalytic activity of CYP P4501A was determined as 7-ethoxyresorufin-O-deethylase (EROD) activity. Directly after PCB exposure and after starvation, EROD activities in liver, gill and head kidney increased with higher PCB dose. Intestinal EROD activities were not detectable at any sampling point. Confinement, known to evoke a stress response increased EROD activities in livers (only directly after PCB exposure) and in head kidneys (directly after PCB exposure and after starvation) but only in fish fed the low PCB-diet. We suggest that stressing tilapia induces EROD activities at non-saturating PCB concentrations and that this effect is more pronounced in nourished than in starved fish. These data show that the cytochrome P450 1A response to toxicants in fish is also influenced by non-chemical factors. This should be considered when EROD activities are used as a biomarker for environmental monitoring studies.

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  1. E.S. Quabius

    Present address: Department of Zoology, University of Aberdeen, Aberdeen, UK

  2. H. Segner

    Present address: Centre for Fish and Wildlife Health, Department of Animal Pathology, University of Berne, Switzerland

Authors and Affiliations

  1. Department of Animal Physiology, University of Nijmegen, Toernooiveld 1, NL-6525, ED Nijmegen, The Netherlands

    E.S. Quabius & S.E. Wendelaar Bonga

  2. Department of Zoology, University of Aberdeen, Aberdeen, UK

    D.T. Nolan

  3. Department of Chemical Ecotoxicology, Centre for Environmental Research, Leipzig, Germany

    H. Segner

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Quabius, E., Nolan, D., Segner, H. et al. Confinement stress and starvation modulate the induction of EROD activity after dietary exposure to PCB 126 in the Mozambique tilapia (Oreochromis mossambicus). Fish Physiology and Biochemistry 25, 109–119 (2001). https://doi.org/10.1023/A:1020590726262

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