Abstract
Gallbladder motility is modulated by intrinsiccholinergic neurons. The aims of this study were todetermine: (1) the effect of electric field stimulation(EFS) on guinea pig gallbladder smooth muscle, and (2) the role of calcium channels inmediating neurotransmitter release. Gallbladder musclestrips were studied isometrically in vitro. EFS (1-16Hz, 100 V, 0.5-msec pulse width, 30-sec train duration) was used to activate the intrinsic nerves.Exogenous acetylcholine was also used to directlystimulate the smooth muscle. EFS produced afrequency-dependent contractile response that wascompletely abolished by tetrodotoxin. EFS-induced contractions at 16Hz were suppressed by 84 ± 4% with atropine,whereas hexamethonium had no effect. The L-type calciumchannel blocker, nifedipine, reduced EFS contractions by 51 ± 4%, whereas it reduced contractionsto acetylcholine by only 11 ± 5%. The N-typecalcium channel blocker, omega-conotoxin GVIA, reducedEFS-induced contractions by 22 ± 9%, but did notaffect acetylcholine-induced contractions. EFS-induced contractions of theguinea pig gallbladder are primarily mediated byactivation of postganglionic cholinergic neurons. Theacetylcholine release from these cholinergic neurons is regulated by L- and N-type calcium channels.The inhibitory effect of calcium channel blockers on thegallbladder seen in vivo may be in part related toinhibition of acetylcholine release from the intrinsic cholinergic nerves of thegallbladder.
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Parkman, H.P., Pagano, A.P., Martin, J.S. et al. Electric Field Stimulation-Induced Guinea Pig Gallbladder Contractions (Role of Calcium Channels in Acetylcholine Release). Dig Dis Sci 42, 1919–1925 (1997). https://doi.org/10.1023/A:1018819411992
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DOI: https://doi.org/10.1023/A:1018819411992