Abstract
An anonymous honey bee locus, detectedpreviously with a cloned probe, has HhaI RFLP allelesspecific to African bees or common to both African andEuropean bees. To facilitate identification of thesealleles, this region, 1231, was made analyzable with thePCR. The two halves of the region, excluding thetermini, were amplified as two overlapping segments.Restriction sites were mapped, and the site differences responsible for the allelic RFLP patterns weredetermined. In the first half of the region, twopolymorphic HhaI sites are present in the commonalleles, whereas one, the other, or both of the sitesare absent in the African alleles. In the secondhalf, a third polymorphic HhaI site is present or absentin both common and African alleles. A short part of thesecond half of the region, including more of the terminus, was amplified as a third segment.Within this segment, close to this terminus, a fourthpolymorphic HhaI site is absent in some Africanalleles.
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Hall, H.G. PCR Amplification of a Locus with RFLP Alleles Specific to African Honey Bees. Biochem Genet 36, 351–361 (1998). https://doi.org/10.1023/A:1018797429804
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DOI: https://doi.org/10.1023/A:1018797429804