Abstract
Besides lactic acid, many lactic acid bacteria also produce proteinaceous metabolites (bacteriocins) such as nisin. As catabolite repression and end-product inhibition limit production of both products, we have investigated the use of alternative methods of supplying substrate and neutralizing or extracting lactic acid to increase yields. Fed-batch fermentation trials using a stillage-based medium with pH control by NH4OH resulted in improved lactic acid (83.4 g/l, 3.18 g/l/h, 95% yield) and nisin (1,260 IU/ml, 84,000 IU/l/h, 14,900 IU/g) production. Removing particulate matter from the stillage-based medium increased nisin production (1,590 IU/ml, 33,700 IU/g), but decreased lactic acid production (58.5 g/l, 1.40 g/l/h, 96% yield). Removing lactic acid by ion exchange resins stimulated higher lactic acid concentrations (60 to 65 g/l) and productivities (2.0 to 2.6 g/l/h) in the filtered stillage medium at the expense of nisin production (1,500 IU/ml, 25,800 IU/g).
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References
Davison, B.H. & Scott, C.D. 1992 A proposed biparticle fluidized-bed for lactic acid fermentation and simultaneous adsorption. Biotechnology and Bioengineering 39, 365-368.
Davison, B.H. & Thompson, J.E. 1992 Simultaneous fermentation and separation of lactic acid in a biparticle fluidized-bed bioreactor. Applied Biochemistry and Biotechnology 34/35, 431-439.
De Vuyst, L., De Poorter, G. & Vandamme, E. 1990 Metabolic control of nisin biosynthesis in Lactococcus lactis subsp. lactis. In Fermentation Technologies: Industrial Applications, ed Yu, P.L. pp. 166-172. New York: Elsevier Applied Science.
De Vuyst, L. & Vandamme, E.J. 1992 Influence of the carbon source on nisin production in Lactococcus lactis subsp. lactis batch fermentations. Journal of General Microbiology 138, 571-578.
Evangelista, R.L., Mangold, A.J. & Nikolov, Z.L. 1994 Recovery of lactic acid by sorption. Applied Biochemistry and Biotechnology 45/46, 131-144.
Gilliland, S.E. 1986 Bacterial starter cultures for foods. Boca Raton, Florida: CRC Press
Hawley, H.B. 1955 The development and use of nisin. Journal of Applied Bacteriology 18, 388-395.
Hirsch, A. 1951 Growth and nisin production of a strain of Streptococcus lactis. Journal of General Microbiology 5, 208-221.
Hurst, A. 1981 Nisin. Advances in Applied Microbiology 27, 85-123.
Hurst, A. & Dring, G.J. 1968 The relation of the length of lag phase of growth to the synthesis of nisin and other basic proteins by Streptococcus lactis grown under different cultural conditions. Journal of General Microbiology 50, 383-390.
Srivastava, A., Roychoudhury, P.K. & Sahai, V. 1992 Extractive lactic acid fermentation using ion-exchange resin. Biotechnology and Bioengineering 39, 607-613.
Tramer, J. & Fowler, G.G. 1964 Estimation of nisin in foods. Journal of the Science of Food and Agriculture 8, 522-528.
Wolf, C.E. 1992. Production and quantification of the bacteriocin nisin from Lactococcus lactis subsp. lactis ATCC 11454. M.S. Thesis, Brookings, SD: South Dakota State University.
Wolf, C.E. & Gibbons, W.R. 1996 Improved method for quanti-fication of the bacteriocin nisin. Journal of Applied Bacteriology 80, 453-457.
Yabannavar, V.M. & Wang, D.I.C. 1991 Extractive fermentation for lactic acid production. Biotechnology and Bioengineering 37, 1095-1100.
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Hul, J.V., Gibbons, W. Neutralization/recovery of lactic acid from Lactococcus lactis: effects on biomass, lactic acid, and nisin production. World Journal of Microbiology and Biotechnology 13, 527–532 (1997). https://doi.org/10.1023/A:1018509207939
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DOI: https://doi.org/10.1023/A:1018509207939