Abstract
A partial sequence of Oat mosaic virus (OMV) has been obtained for four isolates of the virus from four European countries. This represents the first available sequence data for this important disease of winter-sown oats. The longest clone of 1699 nucleotides was obtained from infected English oats using a degenerate primer, designed to members of the Potyviridae family. Alignment of the predicted amino acid sequence with members of the Potyviridae showed closest identity with viruses of the Bymovirus genus. The predicted amino acid sequence has one open reading frame corresponding to part of the NIb and capsid protein, with a 3′ untranslated region of 351 nucleotides, followed by a poly(A) tail. PCR primers were designed to the coat protein and NIb gene of members of the Bymovirus genus and used to obtain partial sequences of 1441 nucleotides at the 3′ end of infected oats from both Wales and France. A specific primer set designed to the English isolate was used to generate a product of 701 nucleotides from OMV-infected oat leaves from Ireland. All four isolates are highly conserved at the amino acid level.
Similar content being viewed by others
References
Anon (1999) Oats in a New Era. Semundo Limited, 49 North Road, Great Abington. Cambridge CB1 6AS, p 54
Brunt AA, Crabtree K, Dallwitz MJ, Gibbs AJ, Watson L and Zurcher EJ (eds) (1996 onwards) ‘Plant Viruses Online: Descriptions and Lists from the VIDE Database’. URL http://biology.anu.edu.au/Groups/MES/vide/
Catherall PL and Hayes JD (1970) Oat Mosaic Virus. Plant Pathology 19: 78-81
Clover GRG and Henry CM (1999) Detection and discrimination of wheat spindle streak mosaic virus and Wheat yellow mosaic virus using multiplex RT-PCR. European Journal of Plant Pathology 105: 891-896
Dinant S, Lot H, Albouy J, Kuziak C, Meyer M and Astiermanifacier S (1991) Nucleotide-sequence of the 3-terminal region of Lettuce mosaic potyvirusRNAshows aGLN/VAL dipeptide at the cleavage site between the polymerase and coat protein. Archives of Virology 116: 235-252
Elliott LG and Lommel SA (1989) Identification and partial characterization of Oat mosaic and Oat golden stripe viruses co-infecting oats in the south-eastern United States. International Working Group on Plant Viruses with Fungal Vectors: First Symposium Proceedings 131-134
Gibbs A and Mackenzie A (1996) A primer pair for amplifying part of the genome of all potyvirids by RT-PCR. Journal of Virological Methods 63: 9-16
Hariri D, Lapierre H, Filleur S, Ploive C and Delaunay T (1996) Production and characterization of monoclonal antibodies to Barley yellow mosaic virus and their use in detection of four bymoviruses. Journal of Phytopathology 102: 283-292
Hebert TT and Panizo CH(1975) Oat MosaicVirus C.M.I/A.A.B. Descriptions of Plant Viruses. No. 145
Kavanagh JA (1990) Soil-borne viruses of winter oats. University College Dublin, Faculty of General Agriculture Research Report 103-104
Kavanagh JA and Lahert H (1990) Soil-borne viruses in winter oats. University College Dublin, Faculty of General Agriculture Research Report 169
Koonin EV (1991) The phylogeny of RNA-dependent RNA polymerases of positive-strand RNA viruses. Journal of General Virology 72: 2197-2206
Macfarlane I, Jenkins JEE and Melville SC (1968) A soil-borne virus of winter oats. Plant Pathology 17: 167-170
McKinney HH (1946) Mosaics of winter oats induced by soilborne viruses. Phytopathology 36: 359-369
Rubies-Autonell C (1992) Una nueva sindrome viral de la avena en Italia. Proceedings of the ‘VI Congreso Latino Americano de Fitopatologia, VI Congreso Nacional de la Sociedad Espanola de Fitopatologia’, Torremolinos 85
Saitou N and Nei M (1987) The Neighbor-joining method - a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4: 406-425
Usugi T and Saito Y (1981) Purification and some properties of Oat mosaic virus. Annuals of the Phytopathology Society of Japan 47: 581-585
Usugi T, Kashiwazak S, Omura T and Tsuchizaki T (1989) Some properties of nucleic acids and coat proteins of soil-borne filamentous viruses. Annuals of the Phytopathology Society of Japan 55: 26-31
Zheng Jianhua and Kavanagh JA (1990) The detection of OMV and OGSV in soil. University College Dublin, Faculty of General Agriculture Research Report 169-171
Author information
Authors and Affiliations
Additional information
The first two authors contributed equally to the work
Rights and permissions
About this article
Cite this article
Monger, W.A., Clover, G.R. & Foster, G.D. Molecular Identification of Oat Mosaic Virus as a Bymovirus. European Journal of Plant Pathology 107, 661–666 (2001). https://doi.org/10.1023/A:1017950215034
Issue Date:
DOI: https://doi.org/10.1023/A:1017950215034