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Induction of tuberisation in vitro with jasmonic acid and sucrose in an Australian terrestrial orchid, Pterostylis sanguinea

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Abstract

Effectsof jasmonic acid (JA) and sucrose on tuber formation were studied in a commontuberous terrestrial orchid Pterostylis sanguinea D.L.Jones& M. Clements (dark banded greenhood orchid) from southwestern Australia.Seeds were germinated symbiotically in vitro on an oatmealagar (OMA) in the presence of a mycorrhizal fungus isolated from the orchidhost. Ten week-old seedlings were transferred into culture vessels containingOMA supplemented with JA (at concentrations 0, 0.1, 1, 5 or 10μM), sucrose (at concentrations 0, 5, 10 or 20 gl−1) or combinations of each. Tuber development in alltreatments occurred approximately twenty-six weeks after seed sowing. Theaddition of 5 or 10 g l−1 sucrose with JA to themedia resulted in higher frequencies of tuber formation in comparison to thecontrol. Significantly higher proportion of seedlings produced tuber when themedium was supplemented with 5 g l−1 sucrose incombination with 5 μM JA, compared to the control.In vitro tuber formation of Pterostylissanguinea can be improved by combined addition of appropriate levelsof JA and sucrose to OMA, which will aid in rapid in vitrotuberisation of terrestrial orchids.

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References

  • Abdala G., Castro G., Guinazu M.M., Tizio R. and Miersch O. 1996. Occurrence of jasmonic acid in organs of Solanum tuberosum L. and its effect on tuberisation. Plant Growth Regul. 19: 139–143.

    Google Scholar 

  • Abe M., Shibaoka H., Yamane Y. and Takahashi N. 1990. Cell cycle-dependent disruption of microtubules by methyl jasmonate in tobacco Y-2 cells. Protoplasma 156: 1–8.

    Google Scholar 

  • Brown A., Thomson-Dans C. and Marchant N. 1998. Western Australia's Threatened Flora. Department of Conservation and Land Management, Perth, Western Australia.

    Google Scholar 

  • Castro G., Kraus T. and Abdala G. 1999. Endogenous jasmonic acid and radial cell expansion in buds of potato tubers. J. Plant Physiol. 155: 706–710.

    Google Scholar 

  • Clements M.A. and Ellyard R.K. 1979. The symbiotic germination of Australian terrestrial orchids. American Orchid Society Bulletin 48: 810–816.

    Google Scholar 

  • Collins M.T. and Dixon K.W. 1992. Micropropagation of an Australian terrestrial orchid, Diuris longifolia R.Br. Aust. J. Exp. Agr. 32: 131–135.

    Google Scholar 

  • Creelman R.A. and Mullet J.E. 1997. Biosynthesis and action of jasmonates in plants. Annu. Rev. Plant Physiol. 48: 355–381.

    Google Scholar 

  • Dixon K. 1991. Seeder/clonal concepts in Western Australian orchids. In: Wells T.C.E. and Willems J.H. (eds), Population Ecology of Terrestrial Orchids. SPB Academic Publishing, Hague, Netherlands, pp. 111–123.

    Google Scholar 

  • Hadley G. 1970. Non-specificity of symbiotic infection in orchid mycorrhiza. New Phytol. 69: 1015–1023.

    Google Scholar 

  • Jasik J. and Mantell S.H. 2000. Effects of jasmonic acid and its methylester on in vitro microtuberisation of three food yam (Dioscorea) species. Plant Cell Rep. 19: 863–867.

    Google Scholar 

  • Keller E.R.J. 1993. Sucrose, cytokinin and etylene influence formation of in vitro bulblets in onion and leek. Genet Res. Crop. Evol. 40: 113–120.

    Google Scholar 

  • Koda Y. 1992. The Role of Jasmonic Acid and Related Compounds in the Regulation of Plant Development. Academic Press Inc., London, UK.

    Google Scholar 

  • Koda Y. 1997. Possible involvement of jasmonates in various morphogenic events. Physiol. Plant 100: 639–646.

    Google Scholar 

  • Koda Y., Takahashi K. and Kikuta Y. 1994. Involvement of jasmonic acid and related compounds in tuberisation of Jerusalem artichoke plants (Helianthus tuberosus L.). Jpn. J. Crop. Sci. 63: 333–338.

    Google Scholar 

  • Matsuki T., Tazaki H. and Fujimori T. 1992. The influence of jasmonic acid methyl ester on microtubules in potato cells and formation of potato tubers. Biosci. Biotech. Biochem. 36: 1329–1330.

    Google Scholar 

  • Miersch O., Regvar M. and Wasternack C. 1999. Metabolism of jasmonic acid in Pisolithus tinctorius cultures. Phyton: Annales Rei Botanicae 39: 243–247.

    Google Scholar 

  • Mohamed-Yasseen Y., Splittstoesser W.E. and Litz R.E. 1994. In vitro shoot proliferation and production of sets from garlic and shallot. Plant Cell Tissue Organ. Cult. 36: 243–247.

    Google Scholar 

  • Myers N., Mittermeier R.A., Mittermeier C.G., dF G.A.B. and Kent J. 2000. Biodiversity hotspots conservation priorities. Nature 403: 853–858.

    Google Scholar 

  • Pelacho A. and Mingo-Castel A.M. 1991. Jasmonic acid induces tuberisation of potato stolons cultured in vitro. Plant Physiol. 97: 1253–1255.

    Google Scholar 

  • Pridgeon A.M. 1996. Orchids-Status Survey and Conservation Action Plan. Gland, Switzerland and Cambridge. IUCN, UK.

    Google Scholar 

  • Pridgeon A.M. and Chase M.W. 1995. Subterranean axes in tribe Diurideae (Orchidaceae): morphology, anatomy and systematics significance. Amer. J. Bot. 82: 1473–1495.

    Google Scholar 

  • Ramsay R.R., Dixon K.W. and Sivasithamparam K. 1986. Patterns of infection and endophytes associated with Western Australian orchids. Lindleyana 1: 203–214.

    Google Scholar 

  • Rasmussen H.N. 1995. Terrestrial Orchids from Seed to Mycotrophic Plant. University Press, Cambridge.

    Google Scholar 

  • Ravnikar M., Vilhar B. and Gogala N. 1992. Stimulatory effect of jasmonic acid on potato stem node and protoplast culture. J. Plant Growth Regul. 11: 29–33.

    Google Scholar 

  • Ravnikar M., Zel J., Plaper I. and Spacapan A. 1993. Jasmonic acid stimulates shoot and bulb formation of garlic in vitro. J. Plant Growth Regul. 12: 73–77.

    Google Scholar 

  • Regvar M. and Gogala N. 1996. Changes in root growth patterns of (Picea abies) spruce roots by inoculation with an ectomycorrhizal fungus Pisolithus tinctorius and jasmonic acid treatment. Trees-Struct. Funct. 10: 410–414.

    Google Scholar 

  • Regvar M., Gogala N. and Zalar P. 1996. Effects of jasmonic acid on mycorrhizal Allium sativum. New Phytol. 134: 703–707.

    Google Scholar 

  • Santos I. and Salema R. 2000. Promotion by jasmonic acid of bulb formation in shoot cultures of Narcissus triandrus L. Plant Growth Regul. 30: 133–138.

    Google Scholar 

  • Sembdner G. and Parthier B. 1993. The biochemistry and the physiological and molecular actions of jasmonates. Annu. Rev. Plant Physiol. 44: 569–589.

    Google Scholar 

  • Shibaoka H. 1994. Plant hormone-induced changes in the orientation of cortical microtubules: Alteration of the cross-linking between microtubules and the plasma membrane. Annu. Rev. Plant Physiol. 45: 527–544.

    Google Scholar 

  • Takahashi K., Fujino K., Kikuta Y. and Koda Y. 1994. Expansion of potato cells in response to jasmonic acid. Plant Sci. 100: 3–8.

    Google Scholar 

  • Takahashi K., Fujino K., Kikuta Y. and Koda Y. 1995. Involvement of the accumulation of sucrose and the synthesis of cell wall polysaccharides in the expansion of potato cells in response to jasmonic acid. Plant Sci. 111: 11–18.

    Google Scholar 

  • Tung P., Hooker T.S., Tampe P.A., Reid D.M. and Thorpe T.A. 1996. Jasmonic acid-effects on growth and development of isolated tomato roots cultured in vitro. Int. J. Plant Sci. 157: 713–721.

    Google Scholar 

  • Warcup J.H. 1973. Symbiotic germination of some Australian terrestrial orchids. New Phytol. 72: 387–392.

    Google Scholar 

  • Zel J., Debeljak N., Ucman R. and Ravnikar M. 1997. The effect of jasmonic acid, sucrose and darkness on garlic (Allium sativum L.cv. ptujski jesenski) bulb formation in vitro. In Vitro Cell Dev. Biol. - Plant 33: 231–235.

    Google Scholar 

  • Zettler L.W. 1997. Terrestrial orchid conservation by symbiotic seed germination: techniques and perspectives. Selbyana 18: 188–194.

    Google Scholar 

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Authors and Affiliations

  1. Biotechnical Faculty, Department of Biology, University of Ljubljana, Vecna pot 111, SI-1000, Ljubljana, Slovenia

    N. Debeljak & M. Regvar

  2. Botanic Gardens and Parks Authority, Science Division, Kings Park & Botanic Garden, Fraser Avenue, Australia

    K.W. Dixon

  3. Faculty of Agriculture, Soil Science and Plant Nutrition, The University of Western Australia, Nedlands, Australia

    K. Sivasithamparam

Authors
  1. N. Debeljak
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  2. M. Regvar
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  3. K.W. Dixon
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  4. K. Sivasithamparam
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Debeljak, N., Regvar, M., Dixon, K. et al. Induction of tuberisation in vitro with jasmonic acid and sucrose in an Australian terrestrial orchid, Pterostylis sanguinea . Plant Growth Regulation 36, 253–260 (2002). https://doi.org/10.1023/A:1016570319387

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