Abstract
Telomeres of the Mamestra brassica holocentric chromosomes were studied by Southern blotting, in-situ hybridization and Bal31 assay evidencing the presence of the telomeric (TTAGG)n repeat. Successively, molecular analysis of telomeres showed that TRAS1 transposable elements were present at the subtelomeric regions of autosomes but not in the NOR-bearing telomeres of the Z and W sex chromosomes. TRAS1 appeared to be transcriptionally active and non-methylated, as evaluated by RT-PCR and digestion with MspI and HpaII. Finally, dot-blotting experiments showed that the 2.8 ± 0.5% of the M. brassicae genome consists of TRAS1.
Similar content being viewed by others
References
Arnaud P, Goubely C, Pelissier T, Deragon M (2000) SINE retroposons can be used in-vivo as nucleation centers for de novo methylation. Mol Cell Biol 20: 3434-3441.
Biessmann H, Mason JM (1994) Telomeric repeat sequences. Chromosoma 103: 154-161.
Biessmann H, Mason JM, Ferry K (1990) Addition of telomere-associated Het-A DNA sequences MhealsM broken chromosome ends in Drosophila. Cell 61: 663-673.
Blackburn EH (1991) Structure and function of telomeres. Nature 350: 569-573.
Cox AV, Bennett ST, Parokonny AS, Kenton A, Callimassia MA, Bennett MD (1993) Comparison of plant telomere locations using a PCR-generated synthetic probe. Ann Botany 72: 239-247.
Donlon TA, Magenis RE (1983) Methyl green is a substitute for distamycin A in the formation of distamycin A/DAPI C-bands. Hum Genet 65: 144-146.
Hochstrasser M, Mathog D, Saumweber H, Sedat JW (1986) Spatial organization of chromosomes in the salivary gland nuclei of Drosophila melanogaster. J Cell Biol 102: 112-123.
Hughes-Schrader S, Schrader F (1961) The kinetochore of the hemiptera. Chromosoma 12: 327-350.
Ijdo JW, Wells RA, Baldini A, Reeders ST( 1991) Improved telomere detection using a telomere repeat probe (TTAGGG)n generated by PCR. NucleicAcids Res. 19: 4780.
Loidl J (1990) The initiation of meiotic chromosome pairing: the cytological view. Genome 33: 759-778.
Luderus ME, van Steensel B, Chong L, Cremers FF, de Lange T (1996) Structure, subnuclear distribution and nuclear matrix association of the mammalian telomeric complex. J Cell Biol 135: 867-881.
Maibeche-Coisne MM, Jacquin-Joly E, Francois MC, Nagnan Le Meillour P (1998) Molecular cloning of two pheromone binding proteins in the cabbage armyworm Mamestra brassicae. Insect Biochem Mol Biol 28: 815-818.
Mandrioli M, Bizzaro D, Gionghi D, Bassoli L, Manicardi GC, Bianchi U (1999) Molecular cytogenetic characterization of a highly repeated DNA sequence in the peach potato aphid Myzus persicae. Chromosoma 108: 436-442.
Mandrioli M, Bizzaro D, Manicardi GC, Bianchi U (2000) The role of rDNA genes during somatic X chromosome association in the aphid Acyrthosiphon pisum. Genome 42: 381-386.
McKee BD, Karpen GH (1990) Drosophila ribosomal RNA genes function as an X-Y pairing site during male meiosis. Cell 61: 61-72.
Meyne J, Ratliff RL, Moyzis RK (1989) Conservation of the human telomere sequence (TTAGGG)n among vertebrates. Proc Natl Acad Sci USA 89: 7049-7053.
Odierna G, Baldanza F, Aprea G, Olmo E (1993) Occurrence of G-banding in metaphase chromosomes of Encarsia berlesei (Hymenoptera: Aphelinidae). Genome 36: 662-667.
Olkazaki S, Tsuchida K, Maekawa H, Ishikawa H, Fujiwara H (1993) Identification of a pentanucleotide telomeric sequence (TTAGG)n in the silkworm Bombyx mori and in other insects. Mol Cell Biol 13: 1424-1432.
Okazaki S, Ishikawa H, Fujiwara H (1995) Structural analysis of TRAS1, a novel family of telomeric repeat-associated retrotransposons in the silkworm, Bombyx mori. Mol Cell Biol 15: 4545-4552.
Palladino F, Laroche T, Gilson A, Axelrode A, Pillus L, Gasser SM (1993) SIR3 and SIR4 proteins are required for the positioning and integrity of yeast telomeres. Cell 75: 543-555.
Pardue ML, DeBaryshe PG (1999) Drosophila telomeres: two transposable elements with important roles in chromosomes. Genetica 107: 189-196.
Pich U, Fuchs J, Schubert I (1996) How do Alliaceae stabilize their chromosome ends in the absence of TTTAGGG sequence? Chromosome Res 4: 207-213.
Pryde FE, Gorharn HC, Louis EJ (1997) Chromosome ends: all the same under their caps. Curr Opin Genet Dev 7: 822-828.
Sahara K, Marec F, Traut W(1999) TTAGG telomeric repeats in chromosomes of some insects and other arthropods. Chromosome Res 7: 449-460.
Salvadori S, Deiana AM, Coluccia E, Floridia G, Rossi E, Zuffardi O (1995) Colocalization of (TTAGGG)n telomeric sequences and ribosomal genes in Atlantic eels. Chromosome Res 3: 54-58.
Schweizer D (1976) Reverse £uorescent chromosome banding with chromomycin and DAPI. Chromosoma 58: 307-324.
Schweizer D, Loidl J (1987) A model for heterochromatin dispersal and the evolution of C-band patterns. In: Stahl A, Luciani J, Vagner-Capodano AM, eds. Chromosomes Today, Vol. 9. London: Allen & Unwin, pp 61-74.
Spence JM, Blackman RL, Testa JM, Ready PO (1998) A 169 bp tandem repeat DNA marker for subtelomeric heterochromatin and chromosomal re-arrangement in aphids of the Myzus persicae group. Chromosome Res 6: 167-175.
Sumner AT( 1972) A simple technique for demonstrating centromeric heterochromatin. Exp Cell Res 75: 304-306.
Traut W, Marec F (1996) Sex chromatin in Lepidoptera. Q Rev Biol 71: 239-256.
White MJ (1973) Animal Cytology and Evolution, 3rd edn. Cambridge: Cambridge University Press, pp 961.
Zhang YJ, Kamnert I, Lopez CC, Cohn M, Edstrom JE (1994) A family of complex tandem DNA repeats in the telomeres of Chironomus pallidivittatus. Mol Cell Biol 14: 8028-8036.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mandrioli, M. Cytogenetic characterization of telomeres in the holocentric chromosomes of the lepidopteran Mamestra brassicae . Chromosome Res 10, 279–286 (2002). https://doi.org/10.1023/A:1016515607278
Issue Date:
DOI: https://doi.org/10.1023/A:1016515607278