biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 45:347-351, 2002 | DOI: 10.1023/A:1016205331021

Direct Differentiation of Shoot Buds from Leaf Explants of Cajanus Cajan L.

P. Misra1
1 Tissue Culture Laboratory, National Botanical Research Institute, Lucknow, India

A protocol was developed for direct differentiation of multiple shoot buds from leaf explants of Cajanus cajan. In a modified Murashige and Skoog's medium supplemented with 2.22 µM benzyladenine (BA), 0.57 µM indole-3-acetic acid (IAA) and 41 µM adenine sulphate (AdS), the segments of basal halves of the first two leaves of a young seedling incubated on filter paper bridges in liquid medium took 20 - 25 d to differentiate shoot buds. The explants after transfer to solidified medium, with lower concentration of BA (0.22 μM) resulted in fast growing healthy shoots. The developed shoots (measuring ca. 3 cm) were rooted in a medium supplemented with 1.42 µM IAA. They were subsequently grown in pots with soil with more than 80 % transplantation success.

Keywords: agar medium; liquid medium; pigeon pea; plant regeneration; histology
Subjects: auxins; Cajanus cajan; cytokinins; in vitro culture, bud and callus induction and growth; in vitro culture, nutrient media modification; in vitro culture, plant regeneration, organogenesis; pigeon pea, differentiation of shoot buds

Published: September 1, 2002  Show citation

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Misra, P. (2002). Direct Differentiation of Shoot Buds from Leaf Explants of Cajanus Cajan L. Biologia plantarum, 45(3), 347-351. doi: 10.1023/A:1016205331021
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