Abstract
Monopartite begomoviral DNAs (2761 bp) were cloned and sequenced from field cotton, okra, and Sida alba, from Gezira, and field okra from Shambat. Comparison of the four apparent full-length begomoviral DNAs revealed 99.3–99.5% shared nucleotide (nt) identity, indicating that they are the same viral species, hereafter, referred to as Cotton leaf curl virus-Sudan (CLCuV-SD). Host range studies revealed that the field okra isolate of CLCuV-SD was whitefly-t ransmissible from okra to okra, M. parviflora, and hollyhock, but not to cotton. In contrast, the cotton isolate of CLCuV-SD infected cotton and hollyhock, but not okra. The genome of CLCuV-SD encodes six open reading frames (ORFs), and was most closely related to other monopartite begomoviruses of the Eastern Hemisphere. CLCuV-SD shared highest nucleotide sequence identity (95.5%) with Okra enation virus (OkEV), but was distantly related (≈74% nt sequence identity) to begomoviruses isolated from cotton in Pakistan. While extensive genomic regions of CLCuV-SD and OkEV are highly conserved (≈99% nt identity), nt sequence identity of the V1 ORF encoding the coat protein was uncharacteristically low (87.9%), suggesting a history of recombination. An analysis conducted with Sawyer's GENECONV program support the recombination hypothesis, indicating that the V1 ORF and a small segment of the intergenic region of CLCuV-SD and OkEV were derived from other begomoviruses. As a BLAST analysis failed to identify a prospective extant source of either V1 ORF, the parental viruses serving as CP donors remain undiscovered or are extinct.
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References
Brown J.K., FAO Plant Prot Bull 39, 5-23, 1990.
Kirkoatrick T.W., Bull Ent Res 12, 323-363, 1931.
Nour A.M. and Nour J.J., Emp Cott Gr Rev 70, 99-103, 1964.
Elnur E. and Abu Salih H.S., PANS 16, 121-131, 1970.
Mansoor S., Bedford I., Pinner M.S., Stanely J., and Markham P.G., J Botany 25, 105-107, 1993.
Nateshan H.M., Muniyappa V., Swanson M.M., and Harrison B.D., Ann Appl Biol 128, 233-244, 1996.
Abdel-Salam A.M., El-Shazly M.A., and Thouvenel J.C., Arab Biotechnol 1(1), 41-58, 1998.
Mansoor S., Khan S.H., Hussain M., Zafar Y., and Pinner M.S., Plant Dis 84, 101, 2000.
Harrison R.D., Liu Y.L., Khalid S., Hamed S., and Otim-Nape G.W., Ann Appl Biol 130, 61-75, 1997.
Sanz A.I., Fraile A., Garcia-Arenal F., Zhou X., and Robinson D.J., J Gen Virol 81, 1839-1849, 2000.
Idris A.M. and Brown J.K., Plant Dis 84, 809, 2000.
Doyle J.J. and Doyle J.L., Phytochem Bull 19, 11-15, 1987.
Ewing B., Hillier L., Wendl M.C., and Green P., Genome Res 8, 175-185, 1998.
Swofford D.L., PAUP * : Phylogenetic Analysis using Parsimony ( * and other Methods). Version 4. Sinauer Assoc. Sunderland, MA, 1998.
Sawyer S., Mol Biol Evol 6, 526-538, 1989.
Padidam M., Sawyer S., and Fauquet C.M., Virology 265, 218-225, 1999.
Arguello-Astorga G.R., Herrera-Estrella L., and Rivera-Bustamante R., Plant Mol Biol 26, 553-556, 1994.
Padidam M., Beachy R.N., and Fauquet C.F., J Gen Virol 76, 249-263, 1995.
Rybicki E.P., Arch Virol 139, 49-77, 1994.
Briddon R.W., Mansoor S., Bedford I.D., Pinner M.S., and Saunders K., Virology 285, 234-243, 2001.
Dry I.B., Rigden J.E., Krake L.R., Mullineaux P.M., and Rezaian A.M., J Gen Virol 74, 147-151, 1993.
Mayo M.A. and Pringle C.R., Virology 79, 649-657, 1998.
Briddon R.W., Mansoor S., Bedford I.D., Pinner M.S., and Markham P.G., Phytopathology 90, S9, 2000.
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Idris, A.M., Brown, J.K. Molecular Analysis of Cotton Leaf Curl Virus-Sudan Reveals an Evolutionary History of Recombination. Virus Genes 24, 249–256 (2002). https://doi.org/10.1023/A:1015380600089
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DOI: https://doi.org/10.1023/A:1015380600089