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Accumulation of Dehydrins and ABA-Inducible Proteins in Wheat Seedlings during Low-Temperature Acclimation

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Abstract

Using one-dimensional SDS-PAGE and immunochemical methods, we detected the presence and estimated the content of dehydrins and ABA-inducible (RAB) polypeptides in etiolated seedlings of four spring and three winter wheat (Triticum aestivum L.) cultivars differing in frost hardiness. We hardened three-day-old seedlings at 4°C for nine days or grew them at 22°C for a day (control seedlings). We established that heat-stable cold-regulated (COR) polypeptides with mol wts of 209, 196, 169, 66, 50, and 41 kD, which are characteristic of hardened wheat seedlings, were homologous to polypeptides from a dehydrin family and polypeptides with mol wts of 209, 196, 66, 50, and 41 kD were immunologically related to RAB-proteins. We supposed that these COR polypeptides were involved in the prevention of local protein dehydration and denaturation during hypothermia. Analysis of the relative content of COR proteins revealed a close correlation between the cultivar frost hardiness and the concentration of these proteins. It seems evident that different accumulation of dehydrins and RAB polypeptides in different cultivars of a single species is one of the causes for different plant frost hardiness.

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REFERENCES

  1. Yamaguchi-Shinozaki, K. and Shinozaki, K., A Novel cis-Acting Element in an Arabidopsis Gene Is Involved in Responsiveness to Drought, Low-Temperature, or High-Salt Stress, Plant Cell, 1994, vol. 6, pp. 251-264.

    Google Scholar 

  2. Close, T.J., Dehydrins: Emergence of a Biochemical Role of a Family of Plant Dehydration Proteins, Physiol. Plant., 1996, vol. 97, pp. 795-803.

    Google Scholar 

  3. Boothe, J.G., Sonnichsen, F.D., de Beus, M.D., and Johnson-Flanagan, A.M., Purification, Characterization, and Structural Analysis of a Plant Low-Temperature-Induced Protein, Plant Physiol., 1997, vol. 113, pp. 367-376.

    Google Scholar 

  4. Thomashow, M.F., Plant Cold Acclimation: Freezing Tolerance Genes and Regulatory Mechanisms, Annu. Rev. Plant Physiol. Plant Mol. Biol., 1999, vol. 50, pp. 571-599.

    Google Scholar 

  5. Jezek, P., Engstova, H., Zackova, M., Vercesi, A.E., Costa, A.D.T., Arruda, P., and Garlid, K.D., Fatty Acid Cycling Mechanism and Mitochondrial Uncoupling Proteins, Biochim. Biophys. Acta, 1998, vol. 1365, pp. 319-327.

    Google Scholar 

  6. Voinikov, V., Pobezhimova, T., Kolesnichenko, A., Var-akina, N., and Borovskii, G., Stress Protein 310 kD Affects the Energetic Activity of Plant Mitochondria under Hypothermia, J. Therm. Biol., 1998, vol. 23, pp. 1-4.

    Google Scholar 

  7. Guy, C.L., Cold Acclimation and Freezing Stress Tolerance: Role of Protein Metabolism, Annu. Rev. Plant Physiol. Plant Mol. Biol., 1990, vol. 41, pp. 187-223.

    Google Scholar 

  8. Yu, X.-M. and Griffith, M., Antifreeze Proteins in Winter Rye Leaves Form Oligomeric Complexes, Plant Physiol., 1999, vol. 119, pp. 1361-1369.

    Google Scholar 

  9. Stupnikova, I.V., Borovskii, G.B., and Voinikov, V.K., Accumulation of Heat-Stable Proteins in Winter Wheat Seedlings during Hypothermia, Fiziol. Rast. (Moscow), 1998, vol. 45, pp. 859-864 (Russ. J. Plant Physiol., Engl. Transl.).

    Google Scholar 

  10. . Stupnikova, I.V., Borovskii, G.B., Antipina, A.I., and Voinikov, V.K., Polymorphism of Thermostable Proteins in Soft Wheat Seedlings during Low-Temperature Acclimation, Fiziol. Rast. (Moscow), vol. 48, pp. 923-929 (Russ. J. Plant Physiol., Engl. Transl.).

  11. . Esen, A.A., A Simple Method for Quantitative, Semi-quantitative and Qualitative Assay of Protein, Anal. Biochem., 1978, vol. 89, pp. 264-273.

    Google Scholar 

  12. . Laemmli, U.K., Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4, Nature, 1970, vol. 227, pp. 680-685.

    Google Scholar 

  13. Timmons, T.M. and Dunbar, B.S., Protein Blotting and Immunodetection, Methods Enzymol., 1990, vol. 182, pp. 679-701.

    Google Scholar 

  14. . Skriver, K., Olsen, F.L., Rogers, J.C., and Mundy, J., Cis-Acting DNA Elements Responsive to Gibberellin and Its Antagonist Abscisic Acid, Proc. Natl. Acad. Sci. USA, 1991, vol. 88, pp. 7266-7270.

    Google Scholar 

  15. . Ono, A., Izawa, T., Chua, N.-H., and Shimamoto, K., The Rab16B Promoter of Rice Contains Two Distinct Abscisic Acid-Responsive Elements, Plant Physiol., 1996, vol. 112, pp. 483-491.

    Google Scholar 

  16. . Marentes, E.M., Griffith, M., Mlynarz, A., and Brush, R.A., Proteins Accumulate in the Apoplast of Winter Rye Leaves during Cold Acclimation, Physiol. Plant., 1993, vol. 87, pp. 499-507.

    Google Scholar 

  17. . Sarhan, F., Ouellet, F., and Vazquez-Tello, A., The Wheat wcs120 Gene Family. A Useful Model to Understand the Molecular Genetics of Freezing Tolerance in Cereals, Physiol. Plant., 1997, vol. 101, pp. 439-445.

    Google Scholar 

  18. . Danyluk, J., Perron, A., Houde, M., Limin, A., Fowler, B., Benhamou, N., and Sarhan, F., Accumulation of an Acidic Dehydrin in the Vicinity of the Plasma Membrane during Cold Acclimation of Wheat, Plant Cell, 1998, vol. 10, pp. 623-638.

    Google Scholar 

  19. . Galau, G.A., Jakobsen, K.S., and Hughes, D.W., The Controls of Late Dicot Embryogenesis and Early Germination, Physiol. Plant., 1991, vol. 81, pp. 280-288.

    Google Scholar 

  20. . Shinozaki, K. and Yamaguchi-Shinozaki, K., Molecular Responses to Drought and Cold Stress, Curr. Opin. Bio-thechnol., 1996, vol. 7, pp. 161-167.

    Google Scholar 

  21. Bray, E.A., Plant Responses to Water Deficit, Trends Plant Sci., 1997, vol. 2, pp. 48-54.

    Google Scholar 

  22. . Hochachka, W. and Somero, G.N., Biochemical Adaptation, Prinston: Prinston Univ. Press, 1984. Translated under the title Biokhimicheskaya adaptatsiya, Moscow: Mir, 1988.

    Google Scholar 

  23. . Wisniewski, M., Close, T.J., Artlip, T., and Arora, R., Seasonal Patterns of Dehydrins and 70-kDa Heat-Shock Proteins in Bark Tissues of Eight Species of Woody Plants, Physiol. Plant., 1996, vol. 96, pp. 496-505.

    Google Scholar 

  24. . Tumanov, I.I., Fiziologiya zakalivaniya i morozostoikosti rastenii (Physiology of Hardening and Frost Resistance of Plants), Moscow: Nauka, 1979.

    Google Scholar 

  25. Peshkova, A.A. and Dorofeev, N.V., Development of Winter Hardiness in Winter Wheat Plants as Dependent on Growth Conditions and the Level of Mineral Nutrition, Agrokhimiya, 1998, no. 6, pp. 26-33.

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Authors and Affiliations

  1. Siberian Institute of Plant Physiology and Biochemistry, Siberian Division, Russian Academy of Sciences, ul. Lermontova 132, Irkutsk, 664033, Russia

    G. B. Borovskii, I. V. Stupnikova, A. I. Antipina & V. K. Voinikov

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  1. G. B. Borovskii
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  2. I. V. Stupnikova
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  3. A. I. Antipina
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  4. V. K. Voinikov
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Borovskii, G.B., Stupnikova, I.V., Antipina, A.I. et al. Accumulation of Dehydrins and ABA-Inducible Proteins in Wheat Seedlings during Low-Temperature Acclimation. Russian Journal of Plant Physiology 49, 229–234 (2002). https://doi.org/10.1023/A:1014857708301

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