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Interleukin-6-producing Cells in a Human Glioblastoma Cell Line are not Affected by Ionizing Radiation

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Abstract

We investigated the production of interleukin 6 (IL-6) by a radioresistant human glioblastoma cell line G5 after single radiation events of 3, 6 and 9 Gy. The total cell number and IL-6 concentration in culture supernatant were assessed 24–96 h after irradiation. The radiation impeded or stopped G5 cell growth in a dose-dependent manner, but unexpectedly did not affect the IL-6 concentration in cell culture media that increased in the same range as in non-irradiated cultures. Furthermore, using flow cytometry, we found that the IL-6 positive cells expansion was unaffected by radiation. These findings suggested that this small (about 1%) fraction of G5 cells, constitutively producing IL-6, is highly radioresistant.

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Authors and Affiliations

  1. Unité d'Immunologie Clinique, Hôpital Gabriel Montpied, France

    Jean-Jacques Dubost & Annie Dosgilbert

  2. Département de Radiothérapie, INSERM U484; Unité de Radiobiologie, France

    Christine Rolhion

  3. Département de Radiothérapie, Unité de Radiobiologie, France

    Andrei Tchirkov & Pierre Verrelle

  4. Centre Léon Bérard, INSERM U 453, Lyon, France

    Suzanne Bertrand

  5. Laboratoire d'Immunologie, Centre Jean Perrin, Clermont-Ferrand, France

    Jacques Chassagne

Authors
  1. Jean-Jacques Dubost
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  2. Christine Rolhion
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  3. Andrei Tchirkov
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  4. Suzanne Bertrand
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  5. Jacques Chassagne
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  6. Annie Dosgilbert
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  7. Pierre Verrelle
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Dubost, JJ., Rolhion, C., Tchirkov, A. et al. Interleukin-6-producing Cells in a Human Glioblastoma Cell Line are not Affected by Ionizing Radiation. J Neurooncol 56, 29–34 (2002). https://doi.org/10.1023/A:1014467804488

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  • DOI: https://doi.org/10.1023/A:1014467804488

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