Abstract
Extracellular administration of a membranepermeable model peptide containing the tripeptide sequence,SLV, at the C-terminus to human endothelial and kidney cellsresulted in an induction of caspase-8 (FLICE), the apicalenzyme of the apoptosis cascade. The unmodified or N-terminally SLV-tagged peptide had no effect, therebyeliminating an unspecific induction of apoptosis as the causeof the caspase activity observed. Drastic alterations ofprimary structure and structure forming properties of thecarrier peptide did not significantly influence the caspase-8inducing activity of the C-terminal SLV-tag, supportingprevious findings that translocation into the cell interior isa more general ability of peptides.
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Scheller, A., Melzig, M. & Oehlke, J. Induction of caspase-8 in human cells by the extracellular administration of peptides containing a C-terminal SLV sequence. Letters in Peptide Science 8, 29–34 (2001). https://doi.org/10.1023/A:1014218104739
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DOI: https://doi.org/10.1023/A:1014218104739