Abstract
One of the important factors responsible for recalcitrance of maize tissue towards Agrobacterium-mediated transformation is the presence of 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one (DIMBOA), an inhibitory metabolite found in maize cells. DIMBOA-resistant strains of Agrobacterium tumefaciens were used to transfer genes coding for GUS (β-glucuronidase) and NPTII (neomycine phosphotransferase II) in maize shoot apical meristems derived from 20 day-old seedlings and immature embryos. GUS expression was higher (21–34%) in the apical meristem and was dependent on the type of infecting strain and explant-age. The PCR analysis of selected tissues confirmed the presence of GUS gene in the transformed cells.
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Mohamalawari, D., Sharma, N.C., Cristae, P. et al. Transformation of maize by 2,4-dihydroxy-7-methoxy-2H-1,4-benzo- xazin-3(4H)-one resistant Agrobacterium strains. Biotechnology Letters 24, 197–203 (2002). https://doi.org/10.1023/A:1014105225659
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DOI: https://doi.org/10.1023/A:1014105225659