Skip to main content
Log in

Lactate removal by anionic-exchange resin improves nisin production by Lactococcus lactis

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

When lactate was removed from sucrose fermentation in situ, using the anionic-exchange resin Amberlite IRA-67, by Lactococcus lactis growing in batch culture, nisin production increased by two-fold when compared to the alkali pH-controlled fermentation. In comparison to sucrose, lactate removal increased nisin production 1.5-fold and 0.3-fold when galactose and glucose were used as carbon sources respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Bibal B, Goma G, Vayssier Y, Pareilleux A (1988) Influence of pH, lactose and lactic acid on the growth of Streptococcus cremoris: a kinetic study. Appl. Microb. Biotechnol. 28: 340-344.

    Google Scholar 

  • De Vuyst L (1995) Nutritional factors affecting nisin production by Lactococcus lactis subsp. lactis NIZO 22186 in a synthetic medium. J. Appl. Bacteriol. 78: 28-33.

    Google Scholar 

  • De Vuyst L, Vandamme EJ (1992) Influence of the carbon source on nisin production in Lactococcus lactis subsp. lactis batch fermentations. J. Gen. Microbiol. 138: 571-578. 64

    Google Scholar 

  • De Vuyst L, Vandamme EJ (1993) Influence of the phosphorus and nitrogen source on nisin production in Lactococcus lactis subsp. lactis batch fermentations using a complex medium. Appl. Microb. Biotechnol. 40: 17-22.

    Google Scholar 

  • De Vuyst L, De Poorter G, Vandamme E (1990) Metabolic control of nisin biosynthesis in Lactococcus lactis subsp. lactis. In: Yu PL, ed. Fermentation Technologies: Industrial Applications. Amsterdam: Elsevier Applied Science, pp. 166-172.

    Google Scholar 

  • Delves-Broughton J, Blackburn P. Evans RJ, Hugenholtz J (1996) Applications of the bacteriocin, nisin. Antonie van Leeuwenhoek 69: 193-202.

    Google Scholar 

  • Gasson MJ (1984) Transfer of sucrose fermenting ability, nisin resistance and nisin production into Streptococcus lactis 712. FEMS Microbiol. Lett. 21: 7-10.

    Google Scholar 

  • Helander IM, Von Wright A, Mattila-Sandholm T-M (1997) Potential of lactic acid bacteria and novel antimicrobials against Gram-negative bacteria. Trends Food Sci. Tech. 8: 146-150.

    Google Scholar 

  • Hengstenberg W (1977) Enzymology of carbohydrate transport in bacteria. Curr. Top. Microbiol. Immunol. 77: 97-126.

    Google Scholar 

  • Kim WS, Dunn NW (1997) Stabilization of the Lactococcus lactis nisin production transposon as a plasmid. FEMS Microbiol. Lett. 146: 285-289.

    Google Scholar 

  • Kim WS, Hall RJ, Dunn NW (1997a) The effect of nisin concentration and nutrient depletion on nisin production of Lactococcus lactis. Appl. Microb. Biotechnol. 48: 449-453.

    Google Scholar 

  • Kim WS, Hall RJ, Dunn NW (1997b) Host specificity of nisin production by Lactococcus lactis. Biotechnol. Lett. 19: 1235-1238.

    Google Scholar 

  • Shimizu H, Mizuguchi T, Tanaka E, Shioya S (1999) Nisin production by a mixed-culture system consisting of Lactococcus lactis and Kluyveromyces marxianus. Appl. Environ. Microbiol. 65: 3134-3141.

    Google Scholar 

  • Taniguchi M, Hoshino K, Urasaki H, Fujii M (1994) Continuous production of an antibiotic polypeptide (nisin) by Lactococcus lactis using a bioreactor coupled to a microfiltration module. J. Ferment. Bioeng. 77: 704-708.

    Google Scholar 

  • Terzaghi BE, SandineWE (1975) Improved medium for lactic streptococci and their bacteriophages. Appl. Microbiol. 29: 807-813.

    Google Scholar 

  • Tramer J, Fowler GG (1964) Estimation of nisin in foods. J. Sci. Food Agri. 15: 522-528.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yu, PL., Dunn, N.W. & Kim, W.S. Lactate removal by anionic-exchange resin improves nisin production by Lactococcus lactis . Biotechnology Letters 24, 59–64 (2002). https://doi.org/10.1023/A:1013865502420

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1013865502420

Navigation