Abstract
Cotyledon explants from perennial statice Limonium bellidifolium (Statice caspia Willd.) were cultured on Murashige and Skoog's basal medium (MS) supplemented with various levels of 2,4-D, kinetin and sucrose. Embryogenic calluses developed over a period of 10 days with the highest response at 4.5 μM (1 mg l−1) 2,4-D, 0.5 μM (0.1 mg l−1) kinetin and 117 mM sucrose. Following induction, the calluses were transferred to MS media supplemented with 88 or 117 mM sucrose and 0 or 0.5 μM kinetin. Somatic embryos at the globular, heart-shaped, torpedo, and cotyledonary stages developed. Fully germinated plantlets developed with the best response in medium supplemented with 117 mM sucrose and 0.5 μM kinetin. Direct somatic embryogenesis without a callus phase was observed with some of the cotyledon explants. Induction, maturation and germination of somatic embryos on the optimized media were equally effective using cotyledon, hypocotyl and root explants. Serial sections of L. bellidifolium cotyledon explants cultured for two weeks indicated that pro-embryogenic masses originated from parenchyma cells below the epidermis. Further histological observations of embryogenic calluses confirmed the initiation and development of globular and heart-shaped embryos and repetitive somatic embryogenesis. Ultrastructural observations indicated that the embryogenic cells were less vacuolate with abundant organelles compared to the cells of the explant. This is the first report of somatic embryogenesis in the Plumbaginaceae.
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References
Alizadeh S & Mantell SH (1991) Early cellular events during direct somatic embryogenesis in cotyledon explants of Solanum aviculare Forst. Ann. Bot. 67: 257–263
Aly MAM, Khalil MFM, Moghaeieb REA, Yousef SS & El-Sharkawy AMA (1994) Somatic embryogenesis in berseem (Trifolium alexandrium L.). The influence of genetic background. Egypt J. Genet. Cytol. 23: 131–141
Ammirato PV (1987) Organizational events during somatic embryo-genesis. In: Green CE, Somers DA, Hackett WP & Biesboer DD (eds) Plant Tissue and Cell Culture (pp 57–81). Alan R. Liss Inc., New York.
Amo-Marco YB & Ibanez MR (1998) Micropropagation of Limonium cavanillesii Erben, a threatened statice, from inflorescence stems. Plant Growth Reg. 24: 49–54
Bajaj YPS (1995) Somatic embryogenesis and pattern of peroxidases in white mustard (Sinapis alba L. Brassica hirta Moench.). In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry (pp 267–273). Springer-Verlag, Berlin Heidelberg
Brar DS & Jain SM (1998) Somaclonal variation: Mechanism and applications in crop improvement. In: Jain SM, Brar DS & Ahloowalia BS (eds) Somaclonal Variation and Induced Mutations in Crop Improvement (pp 15–37). Kluwer Academic Publishers, Dordrecht, The Netherlands
Canhoto JM, Mesquita JF & Cruz GS (1996) Ultrastructural changes in cotyledons of pineapple guava (Myrtaceac) during somatic embryogenesis. Ann. Bot. 78: 513–521
Fowke LC (1982) Electron microscopy of protoplasts. In: Wetter LR & Constable F (eds) Plant Tissue Culture Methods (pp 72–76). National Research Council of Canada, Saskatoon
Gamborg OL, Miller HA & Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151–158
Gray DJ, Compton ME, Harrell RC & Cantliffe DJ (1995) Somatic embryogenesis and the technology of synthetic seed. In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry, Vol 30 (pp 126-151). Springer-Verlag, Berlin, Heidelberg
Haccius B (1978) Question of unicellular origin of non-zygotic embryos in callus cultures. Phytomorphology 28: 74–81
Hanson AD, Rathhinasabapathi B, Rivoal J, Burnet M, Dillon MO & Gage DA (1994) Osmoprotective compounds in the Plumbaginaceae: A natural experiment in metabolic engineering of stress tolerance. Proc. Natl. Acad. Sci. USA 91: 306–310
Harada D (1992) How to grow Limonium (pp 22–25). FloraCulture International, Nov-Dec 1992
Harazy A, Leshem B, Cohen A & Rabinowitch HO (1985) In vitro propagation of statice (Limonium sinuatum) as an aid to breeding. HortScience 20: 361–362
Hartmann HT, Kester DE, Davies Jr FT & Geneve RI (1997) Plant Propagation: Principles and Practices. 6th edn. Prentice Hall, New Jersey (p 168)
Kubitzki K (1993) Plumbaginaceae. In: Kubitzki K, Rehwer JG & Bittrich V (eds) The Families and Genera of Vascular Plants (pp 523–530). Springer-Verlag, Berlin
Kunitake H & Mii M (1990) Plant regeneration from cell culture-derived protoplasts of statice (Limonium perezii Hubbard). Plant Sci. 70: 115–120
Lazzeri PA, Hildebrand DF, Sunega J, Williams EE & Collins GB (1988) Soybean somatic embryogenesis: Interactions between sucrose and auxin. Plant Cell Rep. 7: 517–520
Maquoi E, Hanke DE & Deltour R (1993) The effects of abscisic acid on the maturation of Brassica napus somatic embryos: An ultrastructural study. Protoplasma 174: 144–157
Martin C & Perez C (1995) Micropropagation of five endemic species Limonium from the Iberian peninsula. J. Hort. Sci. 70: 97–103
Martin C & Perez C (1992) Multiplication in vitro of Limonium estevei Fdez. Casas. Ann. Bot. 70: 165–167
Matsumoto T, Nako Y, Takahashi C & Asakai A (1997) Induction of in vitro cultured masses of shoot primordia of hybrid statice and its cryopreservation by vitrification. HortScience 32: 309–311
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant 15: 473–497
Parrot WA, Merkle SA & Williams EG (1991) Somatic embryogenesis: potential for use in propagation and gene transfer systems. In: Murray DR (ed) Advanced Methods in Plant Breeding and Biotechnology (pp 158–200). CAB International, Wallingford
Rathinasabapathi B, Sigua C, Ho J & Gage DA (2000) Osmo-protectant β-alanine betaine synthesis in the Plumbaginaceae: S-adenosyl-L-methionine dependent N-methylation of β-alanine to its betaine is via N-methyl and N, N-dimethyl β-alanines. Physiol. Plant 109: 225–231
Reynolds ES (1963) The use of lead citrate at high pH as an electron opaque stain for electron microscopy. J. Cell Biol. 17: 208–213
Samoylov VM, Tucker DM & Parrot WA (1998) Soybean [Glycine Max (L.) Merrill] embryogenic cultures: The role of sucrose and total nitrogen content on proliferation. In Vitro Cell. Dev. Biol. - Plant 34: 8–13
Seelye J, Maddocks DJ, Burge GK & Morgan ER (1994) Shoot regeneration from leaf discs of Limonium perigrinum using thidiazuron. New Zeal J. Crop Hort Sci. 22: 23–29
Vasil IK & Vasil V (1994) In vitro culture of cereal and grasses. In: IK Vasil & TA Thorpe (eds) Plant Cell and Tissue Culture (pp 293–312) Kluwer Academic Publishers, Dordrecht, The Netherlands
Zheng Q, Dessai AP & Prakash CS (1996) Rapid and repetitive plant regeneration in sweetpotato via somatic embryogenesis. Plant Cell Rep. 15: 381–385
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Aly, M.A., Rathinasabapathi, B. & Kelley, K. Somatic embryogenesis in perennial statice Limonium bellidifolium, Plumbaginaceae. Plant Cell, Tissue and Organ Culture 68, 127–135 (2002). https://doi.org/10.1023/A:1013803031374
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DOI: https://doi.org/10.1023/A:1013803031374