Photosynthetica 2001, 39(1):59-65 | DOI: 10.1023/A:1012491802184

Effect of Iron Deficiency Induced Changes on Photosynthetic Pigments, Ribulose-1,5-Bisphosphate Carboxylase, and Photosystem Activities in Field Grown Grapevine (Vitis Vinifera L. cv. Pinot Noir) Leaves

M. Bertamini1, N. Nedunchezhian1, B. Borghi1
1 Istituto Agrario di San Michele all' Adige, San Michele all' Adige, Italy

The effect of iron deficiency on photosynthetic pigments, ribulose-1,5-bisphosphate carboxylase (RuBPC), and photosystem activities were investigated in field grown grapevine (Vitis vinifera L. cv. Pinot noir) leaves. The contents of chlorophyll (Chl) (a+b) and carotenoids per unit fresh mass showed a progressive decrease upon increase in iron deficiency. Similar results were also observed in content of total soluble proteins and RuBPC activity. The marked loss of large (55 kDa) and small (15 kDa) subunits of RuBPC was also observed in severely chlorotic leaves. However, when various photosynthetic electron transport activities were analysed in isolated thylakoids, a major decrease in the rate of whole chain (H2O → methyl viologen) electron transport was observed in iron deficient leaves. Such reduction was mainly due to the loss of photosystem 2 (PS2) activity. The same results were obtained when Fv/Fm was evaluated by Chl fluorescence measurements in leaves. Smaller inhibition of photosystem 1 (PS1) activity was also observed in both mild and severely chlorotic leaves. The artificial electron donors, diphenyl carbazide and NH2OH, markedly restored the loss of PS2 activity in severely chlorotic leaves. The marked loss of PS2 activity was evidently due to the loss of 33, 23, 28-25, and 17 kDa polypeptides in iron deficient leaves.

Additional key words: carotenoids; chlorophyll; fluorescence; electron transport; photosystem 1 and 2; proteins; ribulose-1,5-bisphosphate carboxylase

Published: March 1, 2001  Show citation

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Bertamini, M., Nedunchezhian, N., & Borghi, B. (2001). Effect of Iron Deficiency Induced Changes on Photosynthetic Pigments, Ribulose-1,5-Bisphosphate Carboxylase, and Photosystem Activities in Field Grown Grapevine (Vitis Vinifera L. cv. Pinot Noir) Leaves. Photosynthetica, 39(1), 59-65. doi: 10.1023/A:1012491802184
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