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Matrix metalloproteinases expression in HL-60 promyelocytic leukemia cells during apoptosis

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Abstract

Human promyelocytic leukemia HL-60 cells have been used as a model to study both the expression of matrix-metalloproteinases and the mechanisms of programmed cell death. In the present study we examined the expression of these proteases in HL-60 cells stimulated by different apoptotic triggers. As shown by zymography, HL-60 cells released three major isofroms of the matrix-degrading proteases; when the leukemic cells were grown in serum-free conditions, as well as after hyperthermia and methotrexate treatment, we found a significant loss of the constitutive production of the 92 kDa matrix-metalloprotease, with an unequivocable molecular and ultrastructural evidence of programmed cell death. These results suggest that in HL-60 cells the expression/release of matrix metalloproteases can be down-regulated in the presence of the apoptotic-induced alterations, and that the decreased matrix-degrading capacity of this leukemic cell line during apoptosis may reduce its invasive potential.

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Authors and Affiliations

  1. Istituto di Istologia ed Analisi di Laboratorio, Facoltà di Scienze MFN, Università degli Studi, Via E. Zeppi, 61029, Urbino, Italy

    G. Gazzanelli

  2. Centro di Citometria e Citomorfologia, Campus Scientifico, Loc. Crocicchia, 61029, Urbino, Italy

    F. Luchetti & E. Falcieri

  3. Istituto di Scienze Morfologiche, Campus Scientifico, Loc. Crocicchia, 61029, Urbino, Italy

    S. Burattini & S. Papa

  4. Istituto di Istologia ed Analisi di Laboratorio, Facoltà di Scienze MFN, Università degli Studi, Via E. Zeppi, 61029, Urbino, Italy

    F. Mannello

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  1. G. Gazzanelli
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  2. F. Luchetti
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  3. S. Burattini
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  4. F. Mannello
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  5. E. Falcieri
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  6. S. Papa
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Gazzanelli, G., Luchetti, F., Burattini, S. et al. Matrix metalloproteinases expression in HL-60 promyelocytic leukemia cells during apoptosis. Apoptosis 5, 165–172 (2000). https://doi.org/10.1023/A:1009688831531

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