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Y-ligation: An efficient method for ligating single-stranded DNAs and RNAs with T4 RNA ligase

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Abstract

Very efficient ligation ofoligodeoxyribonucleotides was attained through asimple molecular construct, which is composed of onestem and two branches (Y-shape), with use of T4 RNAligase. Single-stranded DNAs (naturally, RNAs also) ofmore than 100 nucleotides (even 800 nts) wereconsiderably ligated, approximately as theoreticallyexpected. Owing to the molecular construct adopted,such a tiny amount of ligation products could beamplified to a sufficient amount by PCR and thenrecovered as single-stranded DNAs. This advantage ofbeing amplifiable is shown to be useful for bothcombinatorial chemistry and evolutionary molecularengineering, which deal with a pool of diversity molecules.

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Authors and Affiliations

  1. Department of Functional Materials Science, Saitama University, 255 Shimo-okubo, Urawa, 338-8570, Japan

    Koichi Nishigaki, Katsuya Taguchi, Yasunori Kinoshita, Takuyo Aita & Yuzuru Husimi

Authors
  1. Koichi Nishigaki
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  2. Katsuya Taguchi
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  3. Yasunori Kinoshita
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  4. Takuyo Aita
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  5. Yuzuru Husimi
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Nishigaki, K., Taguchi, K., Kinoshita, Y. et al. Y-ligation: An efficient method for ligating single-stranded DNAs and RNAs with T4 RNA ligase. Mol Divers 4, 187–190 (1998). https://doi.org/10.1023/A:1009644028931

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  • DOI: https://doi.org/10.1023/A:1009644028931

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