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Potentialities of Quantitative Analysis by High-Performance Liquid Chromatography without Reference Standards of Analytes: Use of Reference Data on UV Spectra for Calculating Calibration Coefficients

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Abstract

It was shown by the examples of simple organic compounds and some medicinal substances that, in the quantitative analysis by the internal standard method in high-performance liquid chromatography (HPLC) with UV detection, one can use calibration coefficients calculated from reference data on UV spectra. Determinations should be performed at several constant wavelengths (for example, 220, 254, 280, and 320 nm) using at least two internal standards (a set of eight samples was proposed) rather than one standard. As can be judged from the estimates obtained, the systematic component of the determination error under these conditions does not exceed the contributions of random errors. The developed approach opens up new opportunities for quantitative determinations by HPLC without reference samples of analytes, instead of which reference data on molar absorptivities are used.

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Authors and Affiliations

  1. Chemistry Research Institute, St. Petersburg State University, Universitetskii prosp. 2, Petrodvorets, 198904, Russia

    I. G. Zenkevich

  2. St. Petersburg Institute of Chemistry and Pharmacology, ul. Professora Popova 14, St. Petersburg, 197376, Russia

    V. M. Kosman

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  1. I. G. Zenkevich
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  2. V. M. Kosman
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Zenkevich, I.G., Kosman, V.M. Potentialities of Quantitative Analysis by High-Performance Liquid Chromatography without Reference Standards of Analytes: Use of Reference Data on UV Spectra for Calculating Calibration Coefficients. Journal of Analytical Chemistry 56, 263–271 (2001). https://doi.org/10.1023/A:1009410707919

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