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Endothelial metalloprotease-disintegrin protein (ADAM) is implicated in angiogenesis in vitro

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Abstract

Recently two metalloproteinase, disintegrin, cysteine proteins (MDCs), also called ADAMs were identified on endothelial cells. However the role of these ADAMs are not defined on these cells. In order to elucidate whether ADAMs associated with endothelial cells could be involved in angiogenesis, we have tested the effect of an inhibitor of ADAM (GL 129471) in models of angiogenesis in vitro. Our results showed that GL 129471 inhibited endothelial cell migration and adhesion and increased the number of cells in the G2/M phase leading to an inhibition of cell proliferation. The effects of GL 129471 are not mimicked by the endogenous matrix metalloproteinase inhibitor TIMP-2. These data suggest that ADAMs may play important role in angiogenesis and could provide a new target for inhibition of angiogenesis in cancers.

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Authors and Affiliations

  1. Inserm U353, Institut d'Hématologie, Hôpital Saint- Louis, Université Paris 7–Denis Diderot, 1 Ave Claude Vellefeaux, F-75475, Paris cedex 10, France

    Véronique Trochon, Annick Thomaidis, He Lu & Claudine Soria

  2. Laboratoire DIFEMA, Faculté de Médecine et de Pharmacie de Rouen, Rouen, France

    Véronique Trochon, Hong Li, Marc Vasse & Claudine Soria

  3. le Centre National de la Recherche Scientifique, UMR 1582, le Laboratoire de Vectoriation et Transfert de Genes, Institut Gustave Roussy, F-94805, Villejuif, France

    Hong Li

  4. Laboratoire de Biologie des tumeurs et du développement, Liège, Belgium

    Francis Frankenne

  5. Laboratoires de Biochmiè et Sainte Marie, Hôtel Dieu, Paris, France

    Jeannette Soria

  6. Hoechst-Marion-Roussel, 102 route de Noisy, F-93235, Romainville, France

    Colin Gardner

Authors
  1. Véronique Trochon
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  2. Hong Li
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  3. Marc Vasse
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  4. Francis Frankenne
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  5. Annick Thomaidis
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  6. Jeannette Soria
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  7. He Lu
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  8. Colin Gardner
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  9. Claudine Soria
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Trochon, V., Li, H., Vasse, M. et al. Endothelial metalloprotease-disintegrin protein (ADAM) is implicated in angiogenesis in vitro. Angiogenesis 2, 277–285 (1998). https://doi.org/10.1023/A:1009206817829

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  • DOI: https://doi.org/10.1023/A:1009206817829

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