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RAPD marker polymorphism among commercial winery yeast strains

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Abstract

Random amplified polymorphic DNA (RAPD) analysis was employed to characterize commercial winery yeast strains currently used in Brazilian industries and to examine the relationship between them. Sixteen strains were subjected to RAPD analysis using eight primers, selected from the 40 primers of the kits B and X of Operon Techn. From the total of 93 scorable bands, 39 (41.9%) were polymorphics. Using the accumulated information of several reactions, all strains under investigation were differentiated. Cluster analysis revealed three groups. Most of the strains (11) were clustered in one group. The highest distance (28%) was obtained with the strain Redstar (Universal Food Corp.).

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Authors and Affiliations

  1. Institute of Biotechnology, Universidade de Caxias do Sul, R. Francisco G. Vargas 1130, 95001-970, Caxias do Sul, RS, Brazil

    S. Echeverrigaray, S. Paese-Toresan & J.L. Carrau

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  1. S. Echeverrigaray
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  2. S. Paese-Toresan
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  3. J.L. Carrau
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Echeverrigaray, S., Paese-Toresan, S. & Carrau, J. RAPD marker polymorphism among commercial winery yeast strains. World Journal of Microbiology and Biotechnology 16, 143–146 (2000). https://doi.org/10.1023/A:1008993129713

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  • DOI: https://doi.org/10.1023/A:1008993129713

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