Skip to main content
SpringerLink
Log in

An easy NMR method to study the formation of parallel β-sheets in peptide aggregates

  • Published:
Letters in Peptide Science Aims and scope Submit manuscript

Abstract

There is currently great interest in the study of peptide aggregation by β-sheet formation because of its relevance in pathological states or in the design of self-assembling systems of technological interest. NMR studies of β-sheet aggregates are difficult because of their long correlation times and spectral degeneracy. In this communication we demonstrate the combination of a semiselective TOCSY-NOESY experiment with partial deuterium exchange of labile protons to assign inter-molecular NOE cross peaks and prove the presence of a soluble parallel β-sheet in fast exchange with monomeric Ac-ASTTTNYT-NH2 (Ac-T-NH2) in solution.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Kelly, J.M., Structure, 5 (1997) 595.

    Google Scholar 

  2. Sunde, M. and Blake, C., Adv. Protein Chem., 50 (1997) 123.

    Google Scholar 

  3. Lomakin, A., Chung, D.S., Benedek, G.B., Kirschner, D.A. and Teplow, D.B., Proc. Natl. Acad. Sci. USA, 93 (1996) 1125.

    Google Scholar 

  4. Garzón-Rodriguez, W., SepÚlveda-Becerra, M., Milton, S. and Glabe, C.G., J. Biol. Chem., 272 (1997) 21037.

    Google Scholar 

  5. Aggeli, A., Bell, M., Boden, N., Keen, J.N., Knowles, P.F., McLeish, T.C.B., Pitkeathly, M. and Radford, S.E., Nature, 386 (1997) 259.

    Google Scholar 

  6. Ghadiri, M.R., Kobayashi, K., Granja, J.R., Chadha, R.K. and McRee, D.E., Angew. Chem. Int. Ed. Engl., 34 (1995) 93.

    Google Scholar 

  7. Clark, T.D., Buriak, J.M., Kobayashi, K., Isler, M.P., McRee, D.E. and Ghadiri, M.R., J. Am. Chem. Soc., 120 (1998) 8949.

    Google Scholar 

  8. Wetterberg, L., Alexius, B., Saaf, J., Sonnerborg, A., Britton, S. and Pert, C.B., Lancet 1, (1987) 159.

    Google Scholar 

  9. Farber, E., Cohen, E., Trozak, D. and Wilkinson, D., J. Am. Acad. Dermat., 25 (1991) 658.

    Google Scholar 

  10. Picone, D., Temussi, P.A., Marastoni, M., Tomatis, R. and Motta, A., FEBS Lett., 231 (1988) 159.

    Google Scholar 

  11. Zangger, K. and Sterk, H., Magn. Reson. Chem., 33 (1995) 421.

    Google Scholar 

  12. Tanner, J.E., J. Chem. Phys., 52 (1970) 2523.

    Google Scholar 

  13. Arrondo, J.L., Muga, A., Castresana, J. and Goñi, F., Prog. Biophys. Mol. Biol., 59 (1993) 23.

    Google Scholar 

  14. Pons, M. and Giralt, E., J. Am. Chem. Soc., 113 (1991) 5049.

    Google Scholar 

  15. Burgering, M., Boelens, R. and Kaptein, R., J. Biomol. NMR, 3 (1993) 709.

    Google Scholar 

  16. Bothner-By, A.A. and Gassend, R., Ann. NY Acad. Sci., 222 (1972) 668.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departament de Química Orgànica, Universitat de Barcelona, Martí i Franquès 1-11, E-08028, Barcelona, Spain

    Oscar Millet & Miquel Pons

  2. Departament d'Enginyeria Química, ETSEIB-Universitat Politècnica de Catalunya, Av. Diagonal 647, E-08028, Barcelona, Spain

    Juan J. Pérez

Authors
  1. Oscar Millet
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Juan J. Pérez
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Miquel Pons
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Millet, O., Pérez, J.J. & Pons, M. An easy NMR method to study the formation of parallel β-sheets in peptide aggregates. Letters in Peptide Science 6, 247–253 (1999). https://doi.org/10.1023/A:1008968104503

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1008968104503

Search

Navigation