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Pressure effect on the dynamics of an isolated α-helix studied by 15N-1H NMR relaxation

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Abstract

Dynamics and structure of (1–36)bacteriorhodopsin solubilized in chloroform/methanol mixture (1:1) were investigated by 1H-15N NMR spectroscopy under a hydrostatic pressure of 2000 bar. It was shown that the peptide retains its spatial structure at high pressure. 15N transverse and longitudinal relaxation times, 15N{1H} nuclear Overhauser effects, chemical shifts and the translation diffusion rate of the peptide at 2000 bar were compared with the respective data at ambient pressure [Orekhov et al. (1999) J. Biomol. NMR, 14, 345–356]. The model free analysis of the relaxation data for the helical 9–31 fragment revealed that the high pressure decreases the overall rotation and translation diffusion, as well as apparent order parameters of fast picosecond internal motions (S2 f) but has no effect on internal nanosecond motions (S2 s and τs) of the peptide. The decrease of translation and overall rotation diffusion was attributed to the increase in solvent viscosity and the decrease of apparent order parameters S2 f to a compression of hydrogen bonds. It is suggested that this compression causes an elongation of H-N bonds and a decrease of absolute values of chemical shift anisotropy (CSA). In particular, the observed decrease of S2 f at 2000 bar can be explained by 0.001 nm increase of N-H bond lengths and 10 ppm decrease of 15N CSA values.

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Authors and Affiliations

  1. Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya str., 117871 , Moscow, Russia

    Vladislav Yu. Orekhov, Peter V. Dubovskii & Alexander S. Arseniev

  2. Swedish NMR Centre, Goteborg University, Medicinareg. 5C, P.O. Box 465, SE 405-30, Goteborg, Sweden

    Vladislav Yu. Orekhov

  3. Department of Chemistry, Faculty of Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, 657-8501, Japan

    Hiroaki Yamada & Kazuyuki Akasaka

  4. Graduate School of Science and Technology, Japan

    Kazuyuki Akasaka

Authors
  1. Vladislav Yu. Orekhov
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  2. Peter V. Dubovskii
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  3. Hiroaki Yamada
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  4. Kazuyuki Akasaka
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  5. Alexander S. Arseniev
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Orekhov, V.Y., Dubovskii, P.V., Yamada, H. et al. Pressure effect on the dynamics of an isolated α-helix studied by 15N-1H NMR relaxation. J Biomol NMR 17, 257–263 (2000). https://doi.org/10.1023/A:1008346414720

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