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A Method for Isolation of Total RNA from Fruit Tissues of Banana

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Abstract

We describe a rapid and efficient method for isolation of total RNA from banana fruit tissues. The RNA was extracted with a high ionic strength buffer at room temperature. The proteins, genomic DNA and secondary metabolites in the extract were then removed by precipitation with pre-cooled potassium acetate and repeated phenol/chloroform/isoamyl alcohol extractions. The RNA was recovered by ethanol precipitation. It was relatively free of ribonucleases and was suitable for RT-PCR and northern blot analysis. The procedure can be completed in less than 4 hours.

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Liu, JJ., Goh, CJ., Loh, CS. et al. A Method for Isolation of Total RNA from Fruit Tissues of Banana. Plant Molecular Biology Reporter 16, 87 (1998). https://doi.org/10.1023/A:1007492421119

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  • DOI: https://doi.org/10.1023/A:1007492421119

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